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- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名:
eNOS (49G3) Rabbit mAb
- 抗原:
synthetic peptide corresponding to the sequence of human eNOS
- 应用范围:
W, IP
- 适应物种:
H,B,M,R,Dg
- 保质期:
详见说明书
- 供应商:
CST
- 库存:
大量
- 级别:
详见MSDS文件
- 是否单克隆:
1
- 保存条件:
-20°c
- 规格:
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格: | 产品价格: | ¥请询价 | |
|---|---|---|---|
| 规格: | 100 ul (10 western blots) | 产品价格: | ¥请询价 |
| 规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat B=Bovine Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H B (M) (R) (Dg) | Endogenous | 140 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | eNOS (49G3) Rabbit mAb detects endogenous levels of total eNOS protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human eNOS. |
| Background | Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2 O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验ITGA4(CD49D)信号通路、病理作用与靶向治疗药物研究梳理
整合素alpha 4(ITGA4),又称CD49D,是整合素家族的关键亚基。它通过与β1或β7亚基结合,形成功能性异二聚体α4β1(VLA-4)和α4β7,在免疫细胞的黏附、运输及迁移中扮演核心角色。VLA-4还能介导白细胞在活化内皮细胞上的聚集。鉴于ITGA4在多发性硬化、炎症性肠病等多种自身免疫性疾病的病理过程中发挥关键作用,它已成为治疗干预的重要靶点。 ITGA4的表达分布 ITGA4主要分布于巨核细胞、红细胞、干细胞、NK细胞以及T细胞中。在T细胞亚群中,CD49D主要在CD
:使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。(图 A)使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit,抗体 1:100 稀释;(图 B) 采用普通免疫组化试剂盒,抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注:使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK (Erk1/2)Rabbit mAb 对正常小鼠心脏组织进行免疫
h)即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复:简单易行效果好,CST 推荐使用微波炉完成修复。合适的修复液:根据抗体说明书使用合适的修复液。用柠檬酸修复后,切片需浸泡在修复液中,自然冷却;而用 EDTA 修复后,切片可直接从修复缸中取出,直接进行下一步。注:使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb(#4267),EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
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