Phospho-Tyrosine Mouse mAb (P-

Tyr-102)

价格询价

品牌Cell Signaling Technology
货号
9416
产地USA
更新日期2026年10月11日

应用范围W, IP, IHC-P, F, E-P
适应物种All

美国CST中国公司(丁香通商铺)

品牌商

13 年钻石会员

详细信息
文献和实验
技术资料

抗体英文名：
Phospho-Tyrosine Mouse mAb (P-Tyr-102)
抗原：
synthetic phospho-Tyr-containing peptides
应用范围：
W, IP, IHC-P, F, E-P
级别：
详见MSDS文件
供应商：
CST
保质期：
详见说明书
适应物种：
All
库存：
大量
是否单克隆：
1
保存条件：
-20°c
规格：
100 ug (40 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	100 ug (40 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) F=Flow Cytometry E-P=ELISA (Peptide)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Applications	Reactivity	Sensitivity	Isotype
W IP IHC-P F E-P	All	Endogenous	Mouse IgG1

Protocols	9416: ELISA Peptide , Flow , IHC / Paraffin , Immunoprecipitation , Western Blotting
Specificity / Sensitivity	Phospho-Tyrosine Mouse mAb (P-Tyr-102) is a high affinity IgG1 monoclonal antibody. ELISAs using a wide variety of phospho-peptides show that P-Tyr-102 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence.2D gel western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-102 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-102's fine specificity in terms of the sequence context in which it can recognize phospho-tyrosine seems to differ slightly from that of P-Tyr-100 #9411. P-Tyr-102 does not recognize peptides containing phospho-Ser or phospho-Thr. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Source / Purification	Monoclonal antibody is produced by immunizing animals with synthetic phospho-Tyr-containing peptides . Western Blotting Western blot analysis of extracts from sodium vanadate treated (3 mM for 0.5 hour) NIH/3T3 cells, using Phospho-Tyrosine Mouse mAb (P-Tyr-102). Western Blotting Western blot analysis of extracts from Jurkat cells treated with 1 mM pervanadate for 30 minutes prior to lysis. Proteins were separated by 2D electrophoresis prior to blotting. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Tyrosine Mouse mAb (P-Tyr-102). IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded NCI-H1650 xenograft untreated (left) or lambda-phosphatase-treated (right), using Phospho-Tyrosine Mouse mAb (P-Tyr-102). Flow Cytometry Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or pervanadate-treated (green), using Phospho-Tyrosine Mouse mAb (P-Tyr-102) compared with a nonspecific negative control antibody (red). ELISA-Peptide Phospho-Tyrosine Mouse mAb (P-Tyr-102) ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (Y* denotes phosphorylated tyrosine.)
Background	Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. Schlessinger, J. (2000) Cell 103, 211-25. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.
Application References	Ma, Y.C. et al. (2000) Cell 102, 635-46. Applications: Western Blot Weinberg, J.M. et al. (2001) Am J Pathol 158, 2153-64. Applications: Western Blotting Nakamura, T. et al. (2002) FEBS Lett 521, 190-4. Applications: Western Blotting Weiser, J.N. et al. (2001) Infect Immun 69, 5430-9. Applications: Western Blotting Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
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