- 询价
- Cell Signaling Technology
- 9104
- USA
- 2026年05月28日
- W, IF-F, IF-IC, F
- H,M,R,Mk
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- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
CREB (86B10) Mouse mAb
- 抗原：
recombinant protein specific to the amino terminus of human CREB protein
- 应用范围：
W, IF-F, IF-IC, F
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 库存：
大量
- 适应物种：
H,M,R,Mk
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-F=Immunofluorescence (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IF-F IF-IC F | H M R Mk | Endogenous | 43 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | CREB (86B10) Mouse mAb detects endogenous levels of total CREB protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CREB protein. Western Blotting
Western blot analysis of extracts from 293, COS and PC12 cells, using CREB (86B10) Mouse mAb. Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells, using CREB (86B10) Mouse mAb antibody (blue) compared to a nonspecifc negative control antibody (red). IF-IC
Confocal microscopic images of SK-N-MC cells showing nuclear stain with CREB (86B10) Mouse mAb (red). Blue pseudocolor = DRAQ5 (fluorescent DNA dye). |
| Background | CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ , and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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