- 询价
- Cell Signaling Technology
- 4853
- USA
- 2025年08月01日
- 免疫荧光（IF）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 兔
- 人,大鼠,驴,斑马鱼,细菌,猪
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 抗原来源：
/
- 保质期：
详见说明书
- 适应物种：
人,大鼠,驴,斑马鱼,细菌,猪
- 应用范围：
免疫荧光（IF）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 供应商：
CST
- 抗原：
/
- 宿主：
兔
- 级别：
详见MSDS文件
- 抗体英文名：
COX IV (3E11) Rabbit mAb (Alexa Fluor 488 Conjugate)
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Cytoskeletal Signaling
COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4853
PhosphoSitePlus ® protein, site, and accession data: COX4I1
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-F IF-IC F | H R Mk Z B Pg | Endogenous | Rabbit IgG |
Applications Key: IF-F=Immunofluorescence (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human R=Rat Mk=Monkey Z=Zebrafish B=Bovine Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4853:
- Flow , Immunofluorescence
Specificity / Sensitivity
COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total COX IV protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV. Antibodies were purified by protein A and peptide affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.
Flow Cytometry
Flow cytometric analysis of NIH/3T3 (blue) and Jurkat cells (green) using COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate).
IF-IC
Confocal immunofluorescent analysis of SKOV3 cells using COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red).
IF-F
Confocal immunofluorescent analysis of frozen HCC827 xenograft using COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Note specific staining of human cancer cells. Blue pseudocolor = DRAQ® #4084 (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated COX IV (3E11) Rabbit mAb #4850.
Background
Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.
- Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
- Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
- Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221.
- Barrientos, A. et al. (2002) Gene 286, 53-63.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 4850 COX IV (3E11) Rabbit mAb
- 4844 COX IV Antibody
- 4967 β-Actin Antibody
- 4272 Cytochrome c Antibody
- 2116 β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate)
- 2975 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate)
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验Antibody250µlIF-IC HCA4410SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)Secondary Antibody250µlIF-IC F4412SAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)Secondary Antibody250µlIF-IC F4413SAnti-rabbit IgG (H+L
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
线粒体荧光探针大全：TMRM，Mitotracker，JC-1
valuable for analyzing human mitochondrial myopathies and related disorders.170,173,183,184 Alexa Fluor 488 and Alexa Fluor 594 conjugates of anti�COX subunit I are also available for direct staining of mitochondria (A21296, A21297; Figure 7.79). Mouse
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