- 询价
- Cell Signaling Technology
- 4853
- USA
- 2026年09月24日
- 免疫荧光（IF）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 兔
- 人,大鼠,驴,斑马鱼,细菌,猪
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- 文献和实验
- 技术资料
- 库存：
大量
- 抗原来源：
/
- 保质期：
详见说明书
- 适应物种：
人,大鼠,驴,斑马鱼,细菌,猪
- 应用范围：
免疫荧光（IF）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 供应商：
CST
- 抗原：
/
- 宿主：
兔
- 级别：
详见MSDS文件
- 抗体英文名：
COX IV (3E11) Rabbit mAb (Alexa Fluor 488 Conjugate)
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Cytoskeletal Signaling
COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4853
PhosphoSitePlus ® protein, site, and accession data: COX4I1
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-F IF-IC F | H R Mk Z B Pg | Endogenous | Rabbit IgG |
Applications Key: IF-F=Immunofluorescence (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human R=Rat Mk=Monkey Z=Zebrafish B=Bovine Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4853:
- Flow , Immunofluorescence
Specificity / Sensitivity
COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total COX IV protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV. Antibodies were purified by protein A and peptide affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.
Flow Cytometry
Flow cytometric analysis of NIH/3T3 (blue) and Jurkat cells (green) using COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate).
IF-IC
Confocal immunofluorescent analysis of SKOV3 cells using COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red).
IF-F
Confocal immunofluorescent analysis of frozen HCC827 xenograft using COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Note specific staining of human cancer cells. Blue pseudocolor = DRAQ® #4084 (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated COX IV (3E11) Rabbit mAb #4850.
Background
Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.
- Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
- Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
- Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221.
- Barrientos, A. et al. (2002) Gene 286, 53-63.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 4850 COX IV (3E11) Rabbit mAb
- 4844 COX IV Antibody
- 4967 β-Actin Antibody
- 4272 Cytochrome c Antibody
- 2116 β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate)
- 2975 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate)
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验CST 抗体 9 月特惠｜购买任意 CST 抗体，+168 元即可入手 25μl 标签 / 内参抗体
HRP、Alexa Fluor® 488、Alexa Fluor® 594 等多种标记一抗可选。 热门可选产品参考 货号 产品名称 类型 加价购价格 S0B0005-25u1 B-actin Rabbit mAb (SDT-RO15) 裸抗 168 元 S0B0004-25u1 B-tubulin Recombinant Rabbit mAb (SDT-RO14) 裸抗 168 元 S0B0383-25u1 S-RMab® Myc tag Recombinant Rabbit
-BCMA CAR-T) Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (S-5414) 三、其他CAR 检测试剂 Protein L、抗 Fab多抗属于备选检测试剂，仅推荐用于项目早期预实验筛查，严禁用于GMP批次放行、临床样本检测。 Protein L结合scFv的κ轻链，通用性较强，成本低，适合早期快速预实验初筛；但只能识别κ轻链，λ轻链scFv无法检出，存在检测盲区。 抗Fab多克隆抗体可识别Fab片段，但容易和人内源性IgG发生
Antibody250µlIF-IC HCA4410SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)Secondary Antibody250µlIF-IC F4412SAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)Secondary Antibody250µlIF-IC F4413SAnti-rabbit IgG (H+L
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