- 询价
- Cell Signaling Technology
- 4528
- USA
- 2026年05月27日
- 免疫荧光（IF）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 小鼠
- 人,大鼠,驴
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- 文献和实验
- 技术资料
- 抗体英文名：
Pan-Keratin (C11) Mouse mAb (Alexa Fluor 647 Conjugate)
- 抗原：
/
- 应用范围：
免疫荧光（IF）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 宿主：
小鼠
- 库存：
大量
- 适应物种：
人,大鼠,驴
- 供应商：
CST
- 级别：
详见MSDS文件
- 抗原来源：
/
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Cytoskeletal Signaling
Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) #4528
PhosphoSitePlus ® protein, site, and accession data: K10 , K13 , K18 , K4 , K5 , K6a , K6c , K8 , KRT6B
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC IF-P F | H R Mk | Endogenous | Mouse IgG1 |
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry) IF-P=Immunofluorescence (Paraffin) F=Flow Cytometry
Reactivity Key: H=Human R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4528:
- Flow , Immunofluorescence
Specificity / Sensitivity
Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a cytoskeleton preparation from A431 cells. This antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.
Flow Cytometry
Flow cytometric analysis of Jurkat (blue) and MCF-7 cells (green) using Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate).
IF-IC
Confocal immunofluorescent analysis of MCF-7 cells using Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor) and Histone H3 Antibody #9715 (green).
IF-P
Confocal immunofluorescent analysis of paraffin-embedded H3255 xenograft using Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor) and Histone H3 Antibody #9715 (red).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The unconjugated antibody #4545 reacts with keratins 4, 5, 6, 8, 10, 13 and 18 from human, rat and monkey. CST expects that Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) will also recognize the same keratins in these species.
Background
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
- Moll, R. et al. (1982) Cell 31, 11-24.
- Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
- Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
- Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
- Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
- Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 4548 Keratin 18 (DC10) Mouse mAb
- 4546 Keratin 8/18 (C51) Mouse mAb
- 4545 Pan-Keratin (C11) Mouse mAb
- 4523 Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 488 Conjugate)
- 2985 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate)
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
Antibody250µlIF-IC HCA4410SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)Secondary Antibody250µlIF-IC F4412SAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)Secondary Antibody250µlIF-IC F4413SAnti-rabbit IgG (H+L
组织化学分析。（图 C）使用Anti-Erk1/2 Mouse mAb 鼠单抗进行目的蛋白检测；(图 D) 使用p44/42 MAPK （Erk1/2）Rabbit mAb 兔单抗进行目的蛋白检测。 图 7 免疫组化实验检测Akt表达 注：使用 Anti-Akt (pan) Rabbit mAb 对正常小鼠肝组织进行免疫组织化学分析。（图 E）使用免疫组化试剂盒 M&R HRP/DAB Detection IHC Kit，抗体1:100 稀释；(图F)使用另一种试剂盒，抗体 1:100 稀释。
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