E-Cadherin (24E10) Rabbit mAb

Product Pathways - Adhesion

• pathway
• more info
• application references
• datasheet PDF
• MSDS PDF
• protocols

E-Cadherin (24E10) Rabbit mAb (Alexa Fluor^® 555 Conjugate) #4295

Applications	Reactivity	Sensitivity	Isotype
IF-IC	H M (Dg)	Endogenous	Rabbit IgG

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

4295:

Immunofluorescence

Specificity / Sensitivity

E-Cadherin (24E10) Rabbit mAb (Alexa Fluor^® 555 Conjugate) detects endogenous levels of total E-cadherin protein. The antibody does not cross-react with related family members, such as N-cadherin.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro780 of human E-cadherin protein. The antibody was conjugated to Alexa Fluor^® 555 under optimal conditions with an F/P ratio of 2-6.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 555 fluorescent dye and tested in-house for immunofluroescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated E-Cadherin (24E10) Rabbit mAb #3195.

Background

Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). E-cadherin is considered an active suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).

1. Wheelock, M.J. and Johnson, K.R. (2003) Annu. Rev. Cell. Dev. Biol. 19, 207-235.
2. Christofori, G. (2003) EMBO J. 22, 2318-2323.
3. Hazan, R.B. et al. (2004) Ann. NY Acad. Sci. 1014, 155-163.
4. Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol. 14, 427-434.
5. Rabascio, C. et al. (2004) Cancer Res. 64, 4373-4377.
6. Yamaoka-Tojo, M. et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26, 1991-1997.
7. Patel, I.S. et al. (2003) Int. J. Cancer 106, 172-177.
8. Sanders, D.S. et al. (2000) J. Pathol. 190, 526-530.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

Companion Products

• 3195 E-Cadherin (24E10) Rabbit mAb
• 3199 E-Cadherin (24E10) Rabbit mAb (Alexa Fluor^® 488 Conjugate)

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and 7,429,487) from Epitomics, Inc.

---

For Research Use Only. Not For Use In Diagnostic Procedures.