- 询价
- Cell Signaling Technology
- 4577
- USA
- 2025年10月14日
- W, IP
- H,M
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- 详细信息
- 技术资料
- 抗体英文名：
Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb
- 抗原：
synthetic phosphopeptide corresponding to the sequence surrounding Tyr842 of human FLT3
- 应用范围：
W, IP
- 级别：
详见MSDS文件
- 适应物种：
H,M
- 库存：
大量
- 供应商：
CST
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M | Endogenous | 160 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb detects endogenous levels of FLT3 only when phosphorylated at Tyr842. This antibody may cross-react with other tyrosine-phosphorylated family members. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Tyr842 of human FLT3. Western Blotting
Western blot analysis of cell extracts from SEM cells expressing constitutively activated FLT3 using Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb (upper and middle). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing. Total FLT3 (8F2) Rabbit mAb #3462 shown at the bottom. |
| Background | FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets (3). The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7). Tyr842 is located in the activation loop of FLT3. Mutation of Tyr842 was identified in human AML patients (8).
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| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
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