Ubiquitin (P4D1) Mouse mAb

价格询价

品牌Cell Signaling Technology
货号
3936
产地USA
更新日期2026年05月28日

应用范围W, IHC-P, IF-IC
适应物种All

美国CST中国公司(丁香通商铺)

品牌商

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详细信息
询价记录
文献和实验
技术资料

抗体英文名：
Ubiquitin (P4D1) Mouse mAb
抗原：
1-76 full length bovine ubiquitin
应用范围：
W, IHC-P, IF-IC
供应商：
CST
库存：
大量
级别：
详见MSDS文件
适应物种：
All
保质期：
详见说明书
是否单克隆：
1
保存条件：
-20°c
规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	40 ul (4 western blots)	产品价格：	￥请询价
规格：	100 ul (10 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Applications	Reactivity	Sensitivity	Isotype
W IHC-P IF-IC	All	Endogenous	Mouse IgG1

Protocols	3936: IHC / Paraffin , Immunofluorescence , Western Blotting
Specificity / Sensitivity	Ubiquitin (P4D1) Mouse mAb detects ubiquitin, polyubiquitin and ubiquitinated proteins. This antibody may cross-react with recombinant NEDD8.
Source / Purification	Monoclonal antibody is produced by immunizing animals with 1-76 full length bovine ubiquitin. Western Blotting Western blot analysis of 293 and HeLa cells, untreated or treated with the 26S proteasome inhibitor MG132 (50 µM, 90 minutes), using Ubiquitin (P4D1) Mouse mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded breast carcinoma showing nuclear and cytoplasmic ubiquitin localization, using Ubiquitin (P4D1) Mouse mAb. IF-IC Confocal immunofluorescent analysis of untreated HeLa cells using Ubiquitin (P4D1) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor^® 555 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).
Background	Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH₂ of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25a and Bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (4-7). Ciechanover, A. (1998) EMBO J. 17, 7151-7160. Hochstrasser, M. (2000) Nat. Cell Biol. 2, E153-E157. Hochstrasser, M. (2000) Science 289, 563-564. Bernardi, R. et al. (2000) Oncogene 19, 2447-2454. Aberle, H. et al. (1997) EMBO J. 16, 3797-3804. Salomoni, P. and Pandolfi, P.P. (2002) Nat. Cell Biol. 4, E152-E153. Jesenberger, V. and Jentsch, S. (2002) Nat. Rev. Mol. Cell Biol. 3, 112-121.
Application References	Shelly, M. et al. (2011) Neuron 71, 433-46. Applications: Western Blotting Yamada, K. et al. (2011) J Biol Chem 286, 44057-66. Applications: Western Blotting Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
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