- 询价
- Cell Signaling Technology
- 3556
- USA
- 2025年12月08日
- W
- H,M,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser227 of human RSK2
- 应用范围：
W
- 级别：
详见MSDS文件
- 适应物种：
H,M,R,Mk
- 库存：
大量
- 供应商：
CST
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 90 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb detects endogenous levels of RSK2 only when phosphorylated at Ser227. It shows cross-reactivity with RSK1 when phosphorylated at the homologous serine residues. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser227 of human RSK2. Western Blotting
Western blot analysis of extracts from HeLa, COS and NIH/3T3 cells, treated with lambda-phosphatase or TPA/UV, using Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb. |
| Background | The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3). PDK1 phosphorylates Ser227 in the activation loop of the amino-terminal kinase domain of RSK2, leading to substantial activation of the kinase in vitro and in vivo . The constitutively active PDK1 cooperates with ERK in the activation of RSK following the exposure of cells to growth factors or UV-light (4, 5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
(Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝
TBS 和 5% NGS 中封闭样品。市售的含有酪蛋白的封闭溶液与磷酸化的一抗结合后易减弱信号；因此，我们建议不使用含有酪蛋白的封闭剂进行磷酸化特异性抗体的检测。以上针对封闭步骤所提建议均是在以 SignalStain. Boost IHC Detection Reagent 作为检测试剂的情况下提出的。如果选择使用其他检测试剂，我们则建议使用与二抗来源相同的血清进行封闭。抗体：Phospho–Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718样本：石蜡
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