- 询价
- Cell Signaling Technology
- 3423
- USA
- 2026年05月28日
- IP
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- 文献和实验
- 技术资料
- 抗体英文名：
Rabbit (DA1E) mAb IgG XP Isotype Control (Sepharose Bead Conjugate)__®
- 应用范围：
IP
- 保质期：
详见说明书
- 供应商：
CST
- 级别：
详见MSDS文件
- 库存：
大量
- 标记物：
Sepharose Bead
- 是否单克隆：
0
- 保存条件：
-20°c
- 规格：
400 ul (40 immunoprecipitations)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 400 ul (40 immunoprecipitations) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: IP=Immunoprecipitation
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Isotype |
|---|---|---|
| IP | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Rabbit (DA1E) mAb IgG XP® Isotype Control is not directed against any known antigen. It functions as a control for rabbit IgG monoclonal antibodies. |
| Source / Purification |
IP
Immunoprecipitation of extracts from HeLa cells, untreated or TPA-treated, using Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) (Lanes 1 and 2) or Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Sepharose Bead Conjugate) #3510 (Lanes 3 and 4). The blot was probed using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb #9106. |
| Description | Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (whole molecule) from Cell Signaling Technology is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) is useful to determine non-specific immunoprecipitation complexes. |
| Background | Control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验的染色强度。获得合适的染色强度后，将载玻片置于蒸馏水 (dH2O) 中以阻止进一步显色。抗体：Phospho–Stat3(Tyr705)(D3A7) XP® Rabbit mAb #9145样本：石蜡包埋的人类乳腺肿瘤显色检测：利用 SignalStain® DAB Substrate Kit #8059（左），竞争者提供的 DAB（中和右）CST 的 IHC/IF 明星产品推荐：生化试剂：Catalog NumberProduct NameProduct
as many as 6 million dpm to maximize sensitivity. 4) Put the 100,000 dpm samples aside until step #12. 5) After lyophilization, solubilize 200,000 dpm samples in 350 µl IMP buffer. 6) Add 50 µl of antibody or control (normal rabbit serum, mouse isotype control
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
of antibody or control (normal rabbit serum, mouse isotype control,, etc.) to each tube and incubate overnight for 6 h at room temperature. 7) Add 100 µl of Protein-A-Sepharose solution (shake to suspend slurry before pipetting) and incubate on a tube
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