- 询价
- Cell Signaling Technology
- 3389
- USA
- 2026年01月08日
- W, IP, IHC-P, IF-IC
- H,M,Hm,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
LPP (8B3A11) Mouse mAb
- 抗原：
HIS-tagged recombinant fragment of human LPP
- 应用范围：
W, IP, IHC-P, IF-IC
- 保质期：
详见说明书
- 供应商：
CST
- 适应物种：
H,M,Hm,Mk
- 级别：
详见MSDS文件
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse Hm=Hamster Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC | H M Hm Mk | Endogenous | 75 | Mouse IgG1 |
| Protocols | |
|---|---|
| Specificity / Sensitivity | LPP (8B3A11) Mouse mAb detects endogenous levels of total LPP protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a HIS-tagged recombinant fragment of human LPP. Western Blotting
Western blot analysis of extracts from various cell types using LPP (8B3A110) Mouse mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human small intestine using LPP (8B3A11) Mouse mAb. IF-IC
Confocal immunofluorescent analysis of COS cells using LPP (8B3A11) Mouse mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). |
| Background | LIM-containing lipoma-preferred partner (LPP) belongs to the zyxin family, members of which include LIMD1, ajuba, trip6 and zyxin. Three LIM domains at the carboxy-terminus characterize this family of proteins. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex (1). They shuttle in and out of the nucleus where they may function in transcriptional activation (1).LPP binding partners at cell-cell contacts include the actin regulator α-actinin (2) and the human tumor suppressor scrib which regulates cell migration and polarity (3). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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