- 询价
- Cell Signaling Technology
- 2850
- USA
- 2025年11月05日
- W, IP, IF-IC
- H
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
MSH2 (3A2) Mouse mAb
- 抗原：
recombinant human MSH2
- 应用范围：
W, IP, IF-IC
- 供应商：
CST
- 库存：
大量
- 适应物种：
H
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-IC | H | Endogenous | 100 | Mouse IgG1 |
| Protocols | |
|---|---|
| Specificity / Sensitivity | MSH2 (3A2) Mouse mAb detects endogenous levels of total MSH2 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with recombinant human MSH2. Western Blotting
Western blot analysis of extracts of HeLa and OVCAR3 cells using MSH2 (3A2) Mouse mAb. IF-IC
Confocal immunofluorescent analysis of HeLa cells using MSH2 (3A2) Mouse mAb (green). Actin filaments have been labeled with DY554 phalloidin (red). |
| Background | The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between non-identical DNA sequences and induces both checkpoint and apoptotic responses following certain types of DNA damage (1). MSH2 (MutS homologue 2) forms the hMutS-α dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-α is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins and the Rad50-Mre11-NBS1 complex (2).Mutations in MSH2 have been found in a large proportion of hereditary non-polyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world (3). Mutations have also been associated with other sporadic tumors. |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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