- 询价
- Cell Signaling Technology
- 7803
- USA
- 2025年10月10日
- W
- Rabbit
- All
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Cre Recombinase Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Asp141 of Cre recombinase protein
- 应用范围：
W
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 供应商：
CST
- 适应物种：
All
- 保质期：
详见说明书
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | All | Transfected Only | 38 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Cre Recombinase Antibody recognizes transfected levels of total Cre recombinase protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp141 of Cre recombinase protein. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from 293T cells, mock-transfected (-) or transfected (+) with a Cre recombinase plasmid, using Cre Recombinase Antibody (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower). |
| Background | Cre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34 base pair loxP sites. This reaction can be carried out in vitro , indicating that it does not require accessory factors (3). The Cre/Lox system has been used for a number of in vitro and in vivo applications including targeted gene deletions (4) and gene-specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Regulation of Cre Recombinase: Use of Ligand-Regulated and Dimerizable Cre for Transgenesis
or, if combined with the use of a specific promoter, in a time- and cell-type-dependent manner. The most widely used approach to obtain this regulation relies on the fusion of the recombinase, mostly Cre recombinase, with the ligand-binding domain (LBD
Conditional Gene Knockout Using Cre Recombinase
The directed introduction of null mutations into defined genes has proven invaluable in elucidating gene function in a variety of experimental organisms. In the last decade or so this approach has been extended to mice (1 ) by the combined
Creation and Use of a Cre Recombinase Transgenic Database
Embryonic stem (ES) cell-mediated transgenic approaches have revolutionized mammalian genetics in the last decade. Close to 4000 genes have been targeted to date. Analysis of these genetic alterations has provided an unprecedented
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