- 询价
- Cell Signaling Technology
- 2827
- USA
- 2025年10月01日
- western blot,免疫荧光（IF）,流式细胞（Flow Cyt）
- 兔
- 人
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 抗体英文名：
Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb
- 抗原：
/
- 应用范围：
western blot,免疫荧光（IF）,流式细胞（Flow Cyt）
- 宿主：
兔
- 库存：
大量
- 供应商：
CST
- 抗原来源：
/
- 适应物种：
人
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/300 ul (30 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | 300 ul (30 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Apoptosis
Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb #2827
PhosphoSitePlus ® protein, site, and accession data: Bcl-2
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IF-IC F | H | Endogenous | 28 | Rabbit IgG |
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2827:
- Flow , Immunofluorescence , Western Blotting
Specificity / Sensitivity
Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb detects endogenous levels of Bcl-2 only when phosphorylated at serine 70. The antibody does not cross-react with nonphosphorylated Bcl-2 at endogenous levels or with other Bcl-2 family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding serine 70 of human Bcl-2.
Western Blotting
Western blot analysis of extracts from Jurkat cells, untreated or treated with paclitaxel (1 μM, overnight) and with or without λ phosphatase, using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb (upper) or Bcl-2 #2876 (lower).
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb versus propidium iodide (DNA content).
IF-IC
Immunofluorescent analysis of MCF-7 cells with or without treatment of paclitaxel (1 μM, overnight), using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of SH-SY5Y cells, untreated, using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74 and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway, and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin 3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).
- Murphy, K.M. et al. (2000) Cell Death Differ 7, 102-11.
- Zhu, L. et al. (1999) J Biol Chem 274, 33267-73.
- Maundrell, K. et al. (1997) J Biol Chem 272, 25238-42.
- Yamamoto, K. et al. (1999) Mol Cell Biol 19, 8469-78.
- Ling, Y.H. et al. (1998) J Biol Chem 273, 18984-91.
- Huang, S.T. and Cidlowski, J.A. (2002) FASEB J 16, 825-32.
- Deng, X. et al. (2001) J Biol Chem 276, 23681-8.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 4223 Bcl-2 (D55G8) Rabbit mAb (Human Specific)
- 2870 Bcl-2 (50E3) Rabbit mAb
- 2875 Phospho-Bcl-2 (Thr56) Antibody (Human Specific)
- 9941 Pro-Survival Bcl-2 Family Antibody Sampler Kit
- 6441 SignalSilence® Bcl-2 siRNA I
- 6516 SignalSilence® Bcl-2 siRNA II
- 7071 Phototope® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
