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PCNA (PC10) Mouse mAb
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western blot,免疫沉淀(IP),免疫组化(IHC),免疫荧光(IF)
小鼠
CST
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详见说明书
大量
人,小鼠,大鼠,驴,细菌,猪
详见MSDS文件
1
-20°c
100 ul (20 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (20 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
规格: | 产品价格: | ¥面议 | |
---|---|---|---|
规格: | 100 ul (20 western blots) | 产品价格: | ¥请询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
规格: | 100 ul (20 western blots) | 产品价格: | ¥请询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
PhosphoSitePlus® protein, site, and accession data: PCNA
Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
---|---|---|---|---|
W IP IHC-P IF-IC | H M R Mk B Pg | Endogenous | 36 | Mouse IgG2a |
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey B=Bovine Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
* Product-specific protocol.
PCNA (PC10) Mouse mAb detects endogenous levels of PCNA protein.
Monoclonal antibody is produced by immunizing animals with a recombinant Protein A-PCNA fusion protein obtained from pC2T.
Western blot analysis of human (HeLa), murine (SV-T2), bovine (BAEC), and porcine (PAE) cell extracts using PCNA (PC10) Mouse mAb.
Western blot analysis, using PCNA (PC10) Mouse mAb (upper) and Beta-Actin Antibody #4967(lower), of HeLa cell extract (lane 1) and PCNA immunoprecipitated from the same extract using PCNA (PC10) Mouse mAb (lane 2).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using PCNA (PC10) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing nuclear localization, using PCNA (PC10) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, showing nuclear localization, using PCNA (PC10) Mouse mAb.
Confocal immunofluorescent analysis of NCI-H460 cells using PCNA (PC10) Mouse mAb (green), Phospho-Histone H3 (Ser10) (D2C8) XP Rabbit mAb (Alexa Fluor 555 Conjugate) (red), and p21 Waf1/Cip1 (12D1) Rabbit mAb (blue).
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation and PCNA (PC10) Mouse mAb has been used to assess PCNA levels in hundreds of scientific studies.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
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