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Myc-Tag (9B11) Mouse mAb
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western blot,免疫沉淀(IP),免疫组化(IHC),免疫荧光(IF),流式细胞(Flow Cyt)
小鼠
详见MSDS文件
CST
大量
不限
/
详见说明书
1
-20°c
100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
规格: | 产品价格: | ¥面议 | |
---|---|---|---|
规格: | 100 ul (10 western blots) | 产品价格: | ¥请询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
规格: | 100 ul (10 western blots) | 产品价格: | ¥请询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
Applications | Reactivity | Sensitivity | Isotype |
---|---|---|---|
W IP IHC-P IF-IC F | All | Transfected Only | Mouse IgG2a |
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Myc-Tag (9B11) Mouse mAb detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).
Western blot analysis of cell extracts expressing carboxy-terminal Myc-tagged protein (lane 1), amino-terminal Myc-tagged protein (lane 2) or control cell extracts (lane 3), using Myc-Tag (9B11) Mouse mAb.
Immunoprecipitation of cell extracts alone (lane 1), extracts overexpressing carboxy-terminal Myc-tagged protein (lane 2) or amino-terminal Myc-tagged protein (lane 3), using Myc-Tag (9B11) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded COS cell pellets, control (left) or transfected with a carboxy-terminal Myc tagged protein (right), using Myc-Tag (9B11) Mouse mAb.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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