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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.
- 亚型:
IgG2b
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
HRP
- 适应物种:
Human, Mouse, Rat, Hamster
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX627408-01
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, IHC-P
- 浓度:
0.62 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
GAPDH
- 抗体英文名:
GAPDH antibody [GT239] (HRP)
- 抗体名:
GAPDH 抗体 [GT239] (HRP)
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (HRP) (GTX627408-01) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GAPDH antibody [GT239] (HRP) detects GAPDH antibody protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
GAPDH antibody [GT239] (HRP) (GTX627408-01) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Willis A et al., Mol Neurobiol 2022 (PMID:35233718)
Revollo L. et al., bioRxiv 2021 (Epub)
Klatt D et al., Cells 2020 (PMID:33003308)
Wang L et al., Proc Natl Acad Sci U S A 2020 (PMID:31871155)
Kim DJ et al., J Virol 2020 (PMID:32878886)
Phatarpekar PV et al., J Cell Biol 2020 (PMID:32841357)
Tran DT et al., Diabetes 2020 (PMID:32457219)
Jessen TN et al., Exp Cell Res 2017 (PMID:29097183)
Lu RA et al., Front Pharmacol 2020 (PMID:32435188)
Alex G. Johnson et al., bioRxiv 2019 (Epub)
Ma S et al., Am J Physiol Renal Physiol 2019 (PMID:31339777)
Taura M et al., Proc Natl Acad Sci U S A 2019 (PMID:30670656)
Jessen TN et al., Exp Cell Res 2019 (PMID:30472097)
Schrecengost RS et al., Mol Cancer Res 2015 (PMID:26271487)
Olabisi OA et al., Proc Natl Acad Sci U S A 2016 (PMID:26699492)
Heneghan JF et al., Am J Physiol Cell Physiol 2015 (PMID:26108665)
Escalante-Covarrubias Q et al., Nat Commun 2023 (PMID:36973248)
S?nchez-Ram?rez E et al., J Cell Biol 2022 (PMID:36197339)
Ma S et al., PLoS One 2022 (PMID:36103570)
Taura M et al., Proc Natl Acad Sci U S A 2022 (PMID:35549556)
Revollo L et al., Blood 2022 (PMID:34329392)
Antonino Asaro et al., J Cell Sci 2021 (PMID:34557909)
Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
GAPDH抗体|GAPDH抗体大全-艾美捷指导您购买合适的GAPDH抗体
,但是进口品牌如Abcam、Millipore、CST等价格偏高,一般在¥3000/100ul左右。下面我们为您推荐三款GAPDH原装进口抗体,分别是美国EarthOx和ProteinTech的产品。EarthOx的Anti-GAPDH Monoclonal Antibody (货号E021010 )是小鼠源的单抗,其效价很高,厂商推荐的WB起始稀释比率为1:1000,实际应用过程中,有使用1:10000或更高的报告,远远高于此稀释比率,说明其免疫效价很高,另外,其检测种属也很多,包括人、牛、猪、小鼠
Conjugation of Antibody to HRP
Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki Reagents
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