Human HDAC3 (Histone deacetylase 3), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal region of the protein.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Monoclonal
标记物:
Unconjugated
适应物种:
Human
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX60832
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Mouse
应用范围:
ICC/IF, ChIP assay
靶点:
HDAC3
抗体英文名:
HDAC3 antibody [GT9080]
抗体名:
HDAC3 抗体 [GT9080]
规格:
50 μl
ICC/IF analysis of HeLa cells using HDAC3 antibody (red) and DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC3 antibody at a dilution of 1:500.
ChIP was performed with HeLa extracts and either 5 μg of HDAC3 antibody or control IgG. The precipitated DNA was detected by qPCR with primers targeting to GAPDH promoter, c-fos promoter and the coding region of p21, a known target gene of HDAC3.