Human HDAC3 (Histone deacetylase 3), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal region of the protein.
亚型:
IgG1
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Monoclonal
标记物:
Unconjugated
适应物种:
Human
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX60366
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Mouse
应用范围:
ICC/IF, ChIP assay
浓度:
2 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
HDAC3
抗体英文名:
HDAC3 antibody [GT9007]
抗体名:
HDAC3 抗体 [GT9007]
规格:
50 μg
ICC/IF analysis of 4% PFA fixed HeLa cells using GTX60366 HDAC3 antibody [GT9007]. Red : Primary antibody Blue : DAPI Dilution : 1:500
ChIP analysis of sheared chromatin from 10⁴ HeLa cells using GTX60366 HDAC3 antibody [GT9007]. A titration of the antibody consisting of 1, 2, 5, and 10 μg per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and GAPDH, and for the coding region of p21, a known target gene of HDAC3. Figure 4 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).