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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Protein fragment expressed in E. coli corresponding to amino acids 1759-2093.
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX70137
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
CENPF
- 抗体英文名:
CENPF antibody [14C10 1D8]
- 抗体名:
CENPF 抗体 [14C10 1D8]
- 规格:
100 μl
Confocal immunofluorescence staining (Olympus FV10i) of Mitosin. U2OS cells were fixed by 4% PFA and costained with Mitosin 14C10 1D8 AB (Red; cat# GTX70137; 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB (Green; cat# GTX112141; 1:500 Ab dilution ), a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 um
CENPF antibody [14C10 1D8] detects CENPF protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)
5% SDS-PAGE
CENPF antibody [14C10 1D8] (GTX70137) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
CENPF antibody [14C10 1D8] detects CENPF protein at nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
CENPF antibody [14C10 1D8] (GTX70137) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验Gregson HC et al., Chromosome Res 2002 (PMID:12199140)
Simmen RC et al., DNA Cell Biol 2002 (PMID:11953011)
Abaza A et al., J Biol Chem 2003 (PMID:12740395)
Sorensen CS et al., Mol Cell Biol 2000 (PMID:11003657)
Karja V et al., Hum Pathol 2010 (PMID:19801161)
Konstantinidou AE et al., Histol Histopathol 2003 (PMID:12507285)
of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins. A polyclonal antibody was raised against purified ST-IV. A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti
Introduction of human 14-3-3 proteins
of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins.A polyclonal antibody was raised against purified ST-IV.A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti
14 Immunotargeting of Catalase to the Pulmonary Vascular Endothelium
. In order to provide catalase with such an affinity, catalase could be chemically conjugated with a carrier antibodies recognizing the surface endothelial antigens (8 ). A monoclonal antibody (MAb) against such an antigen, angiotensin-converting enzyme
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