- ￥6100
- GeneTex
- 美国
- GTX60354
- 2025年07月12日
- WB, ICC/IF, Dot, ELISA, ChIP assay
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Histone H3 containing the monomethylated lysine 36 (H3K36me1), using a KLH-conjugated synthetic peptide.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60354
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, Dot, ELISA, ChIP assay
- 浓度：
0.94 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H3K36me1 (Mono-methyl Lys36)
- 抗体英文名：
Histone H3K36me1 (Mono-methyl Lys36) antibody - ChIP grade
- 抗体名：
Histone H3K36me1 (Mono-methyl Lys36) 抗体 - ChIP grade
- 规格：
50 μg
WB analysis of histone extracts (15 μg) from HeLa cells using GTX60354 Histone H3K36me1 (Mono-methyl Lys36) antibody - ChIP grade.
Dilution : 1:1,000
ELISA analysis of peptide containing the histone modification of interest using GTX60354 Histone H3K36me1 (Mono-methyl Lys36) antibody - ChIP grade.
ICC/IF analysis of 4% PFA fixed U2OS cells using GTX60354 Histone H3K36me1 (Mono-methyl Lys36) antibody - ChIP grade. Staining of the cells with the H3K36me1 antibody after incubation of the antibody with 2 ng/μl blocking peptide containing the unmodified and the mono-, di- and trimethylated H3K36 (Figure B, C, D, respectively).
Red : Primary antibody
Blue : DAPI
Dilution : 1:500
Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H3 or the unmodified H3K36 using GTX60354 Histone H3K36me1 (Mono-methyl Lys36) antibody - ChIP grade.
Dilution : 1:20,000
ChIP analysis of sheared chromatin from 1.5x10⁶ U2OS cells using GTX60354 Histone H3K36me1 (Mono-methyl Lys36) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the constitutively expressed GAPDH gene and for the inactive gene MYOD. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
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文献和实验. However, to address in detail what happens at specific sites in vivo, chromatin immunoprecipitation (ChIP) is the method of choice. Here, we describe how ChIP can be performed on non-fixed chromatin from animal cells or tissues (fresh or frozen) to analyze histone
. However, to address in detail what happens at specific sites in vivo, chromatin immunoprecipitation (ChIP) is the method of choice. Here, we describe how ChIP can be performed on non-fixed chromatin from animal cells or tissues (fresh or frozen) to analyze histone
法所替代。利用微球菌核酸酶的切割特性（在染色质核小体之间的 DNA 更容易被切开），可以快速温和地将染色质 DNA 切断，且最大限度地保持了细胞原有染色质结构以及目的蛋白与 DNA 的结合状态，使 ChIP 的准确性和结果可信度大大提高。CST 推出的 SimpleChIP® 酶解法试剂盒，含有标准 ChIP 流程所需的整套试剂、ChIP 级蛋白质 G 微珠、阳性对照抗体（Histone H3(D2B12) XP® Rabbit mAb (ChIP Formulated) #4620）、阴性对照抗体、DNA
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