- ￥5000
- GeneTex
- 美国
- GTX54957
- 2025年07月07日
- WB, IHC-P
- Rabbit
- Human, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of Cyclin D1. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX54957
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Cyclin D1 (phospho Thr286)
- 抗体英文名：
Cyclin D1 (phospho Thr286) antibody
- 抗体名：
Cyclin D1 (phospho Thr286) 抗体
- 规格：
100 μl
WB analysis of EGF-treated MCF7 (A), rat muscle (B) whole cell lysates using GTX54957 Cyclin D1 (phospho Thr286) antibody.
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX54957 Cyclin D1 (phospho Thr286) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
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文献和实验Park SH et al., Oncology Letters 2017
Park SH et al., Oncol Lett 2017 (PMID:29344143)
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
. It has been tested with antibodies against cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326), cyclin D2/D3 (cat. no. 14711A, clone G107-22) and cyclin E Procedure Prepare the cell type of interest as a single cell suspension and wash once with cold PBS
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
. It has been tested with antibodies against cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326), cyclin D2/D3 (cat. no. 14711A, clone G107-22) and cyclin E, and is based on protocols originally designed by Z. Darzynkiewicz and colleagues. Although FITC-conjugated
Disorders of the cell cycle regulatory machinery play a key role in the pathogenesis of cancer. The gene encoding the cyclin D1 protein, a regulator of the progression from the G1- to S-phase, is often found disrupted in the cancer cell
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