A synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Mouse, Rat
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX52341
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ICC/IF, IHC-P, IHC
浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
The sequence around AMPK alpha 1 (phospho Thr183) is conserved with the sequence around AMPK alpha 2 (phospho Thr172).AAPK1
Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody (GTX52341) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
IHC-P analysis of rat spleen tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody. Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer Dilution: 1:100
IHC-P analysis of rat skin tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody. Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer Dilution: 1:100
IHC-P analysis of human colon tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody. Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer Dilution: 1:100
WB analysis of PANC1 cells using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody pre-incubated with phospho- or non-phospho- peptide. P-peptide : phospho-peptide N-peptide : non-phospho-peptide
Western blot analysis of AMPK alpha phosphorylation expression in Heatshock treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
IHC-P analysis of rat lung tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody. Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer Dilution: 1:100
Application Reference
Simanjuntak Y et al., PLoS Pathog 2020 (PMID:32392268)
Lu CC et al., Int J Oncol 2019 (PMID:30720062)
Yang Q et al., Nat Commun 2018 (PMID:30405100)
Yeh YT et al., Nutrients 2017 (PMID:29036927)
Vadhan A et al., Oncol Lett 2023 (PMID:36589668)
Qiuhua Yang et al., Front Cell Dev Biol 2020 (PMID:33511118)
Ilaria Zuliani et al., Int J Mol Sci 2021 (PMID:33916835)
Hsing-I Tseng et al., Mol Oncol 2022 (PMID:35298869)
Lu KY et al., Biochim Biophys Acta Mol Cell Biol Lipids 2021 (PMID:34487913)
Kang DH et al., Oncol Lett 2021 (PMID:33552282)
Zuliani I et al., Neurotherapeutics 2020 (PMID:33258073)