- ￥5300
- GeneTex
- 美国
- GTX52341
- 2025年07月12日
- WB, ICC/IF, IHC-P, IHC
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX52341
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
The sequence around AMPK alpha 1 (phospho Thr183) is conserved with the sequence around AMPK alpha 2 (phospho Thr172).AAPK1
- 抗体英文名：
AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody
- 抗体名：
AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) 抗体
- 规格：
100 μg
Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody (GTX52341) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
IHC-P analysis of rat spleen tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100
IHC-P analysis of rat skin tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100
IHC-P analysis of human colon tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100
ICC/IF analysis of PFA-fixed C2C12 cells using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Red : Primary antibody
Permeabilization : 0.1% saponin
Dilution : 1:400
WB analysis of PANC1 cells using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Western blot analysis of AMPK alpha phosphorylation expression in Heatshock treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
IHC-P analysis of rat lung tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100
ICC/IF analysis of PFA-fixed 293 cells using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Red : Primary antibody
Permeabilization : 0.1% Triton X-100
Dilution : 1:200
IHC-P analysis of human mammary cancer tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100
IHC-P analysis of human colorectal cancer tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100
IHC-P analysis of human gastric cancer tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100
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文献和实验Simanjuntak Y et al., PLoS Pathog 2020 (PMID:32392268)
Lu CC et al., Int J Oncol 2019 (PMID:30720062)
Yang Q et al., Nat Commun 2018 (PMID:30405100)
Yeh YT et al., Nutrients 2017 (PMID:29036927)
Vadhan A et al., Oncol Lett 2023 (PMID:36589668)
Qiuhua Yang et al., Front Cell Dev Biol 2020 (PMID:33511118)
Ilaria Zuliani et al., Int J Mol Sci 2021 (PMID:33916835)
Hsing-I Tseng et al., Mol Oncol 2022 (PMID:35298869)
Lu KY et al., Biochim Biophys Acta Mol Cell Biol Lipids 2021 (PMID:34487913)
Kang DH et al., Oncol Lett 2021 (PMID:33552282)
Zuliani I et al., Neurotherapeutics 2020 (PMID:33258073)
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