alpha Catenin antibody [N1N3]

alpha Catenin antibody [N1N3] detects alpha Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Green: alpha Catenin antibody [N1N3] (GTX111168) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha 1 Catenin antibody [N1N3] detects alpha 1 Catenin protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
alpha 1 Catenin antibody [N1N3] (GTX111168) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Catenin antibody [N1N3] (GTX111168) diluted at 1:500.

alpha 1 Catenin antibody [N1N3] detects alpha 1 Catenin protein at membrane on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
alpha 1 Catenin antibody [N1N3] (GTX111168) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

alpha 1 Catenin antibody [N1N3] immunoprecipitates CTNNA1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of CTNNA1 protein by 4 μg alpha 1 Catenin antibody [N1N3] (GTX111168)
5 % SDS-PAGE
The immunoprecipitated CTNNA1 protein was detected by alpha 1 Catenin antibody [N1N3] (GTX111168) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Sample (30 ug of whole cell lysate)
A: NIH-3T3
5% SDS PAGE
GTX111168 diluted at 1:500

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
5% SDS PAGE
GTX111168 diluted at 1:1000

Immunofluorescence analysis of methanol-fixed HeLa, using alpha Catenin(GTX111168) antibody at 1:500 dilution.