- ￥1700 - 4000
- GeneTex
- 美国
- GTX111168
- 2025年07月14日
- WB, ICC/IF, IHC-P, IP
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the N-terminus region of human alpha Catenin. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX111168
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
alpha Catenin
- 抗体英文名：
alpha Catenin antibody [N1N3]
- 抗体名：
alpha Catenin 抗体 [N1N3]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
alpha Catenin antibody [N1N3] detects alpha Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Green: alpha Catenin antibody [N1N3] (GTX111168) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha 1 Catenin antibody [N1N3] detects alpha 1 Catenin protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
alpha 1 Catenin antibody [N1N3] (GTX111168) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Catenin antibody [N1N3] (GTX111168) diluted at 1:500.
alpha 1 Catenin antibody [N1N3] detects alpha 1 Catenin protein at membrane on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
alpha 1 Catenin antibody [N1N3] (GTX111168) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
alpha 1 Catenin antibody [N1N3] immunoprecipitates CTNNA1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of CTNNA1 protein by 4 μg alpha 1 Catenin antibody [N1N3] (GTX111168)
5 % SDS-PAGE
The immunoprecipitated CTNNA1 protein was detected by alpha 1 Catenin antibody [N1N3] (GTX111168) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Sample (30 ug of whole cell lysate)
A: NIH-3T3
5% SDS PAGE
GTX111168 diluted at 1:500
Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
5% SDS PAGE
GTX111168 diluted at 1:1000
Immunofluorescence analysis of methanol-fixed HeLa, using alpha Catenin(GTX111168) antibody at 1:500 dilution.
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文献和实验Lai F et al., Cell Biosci 2023 (PMID:37024990)
Akin R et al., Int J Mol Sci 2019 (PMID:31847310)
Yuqi Zhang et al., bioRxiv 2019 (Epub)
Biochemical Characterization of a Testis-Predominant Isoform of N-Alpha Acetyltransferase
N-alpha protein acetylation, catalyzed by N-alpha acetyltransferase complex, is a common protein �modification process in eukaryotic cells. Despite its widespread occurrence, the biological significance of this modification process
医生和球 看的文献较少，自己知道的只有wnt、PTEN等，请问还有哪些通路与β-catenin有关，有无类似的综述？谢谢！ 狐狸renart 只知道wnt chuckdouble The canonical Wnt/beta-catenin signalling pathway. Barker N. Methods Mol Biol. 2008;468:5-15
，后来发现与我买的beta-catenin一抗（中杉金桥分装的santa cruz的产品）说明书上的结果一致。请问大家做beta-catenin免疫荧光用的是哪里的抗体啊，或者是我的方法有问题吗？我也用0.5%TritonX-100打孔了，仍不行。我在这里先谢谢各位了！ zhao22840718 还有一种方法不知有没有人试过，就是把beta-catenin构建到pEGFP-N3类载体中，表达融合蛋白，把载体转入细胞，然后加入你的药物。荧光显微镜下观察进核情况
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