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Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.
IgG
Liquid
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Polyclonal
Unconjugated
Human, Mouse, Rat
12 months from the shipping date of the product.
Human
GTX110543
Primary Antibodies
Available
GeneTex
Rabbit
WB, ICC/IF, IHC-P, IHC-Fr, IP, Dot
0.95 mg/ml (Please refer to the vial label for the specific concentration.)
IP/MS validation was supported by references (PMID:30377401)
Caspase 3 antibody
Caspase 3 抗体
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Wild-type (WT) and Caspase 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunoprecipitation of Caspase 3 protein from HeLa whole cell extracts using 5 μg of Caspase 3 antibody (GTX110543).
Western blot analysis was performed using Caspase 3 antibody (GTX110543).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Caspase 3 antibody detects Caspase 3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Caspase 3 stained by Caspase 3 antibody (GTX110543) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Caspase 3 antibody detects Caspase 3 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Caspase 3 antibody (GTX110543) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Caspase 3 antibody detects Caspase 3 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
12% SDS-PAGE
Caspase 3 antibody (GTX110543) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Caspase 3 antibody detects Caspase 3 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Caspase 3 stained by Caspase 3 antibody (GTX110543) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.
Caspase 3 antibody detects Caspase 3 protein by western blot analysis. Un-treated (-) and treated (+, 30 μM Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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