- ￥1700 - 4000
- GeneTex
- 美国
- GTX110543
- 2025年07月16日
- WB, ICC/IF, IHC-P, IHC-Fr, IP, Dot
- Rabbit
- Human, Mouse, Rat
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- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX110543
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, IP, Dot
- 浓度：
0.95 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
IP/MS validation was supported by references (PMID:30377401)
- 抗体英文名：
Caspase 3 antibody
- 抗体名：
Caspase 3 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Wild-type (WT) and Caspase 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunoprecipitation of Caspase 3 protein from HeLa whole cell extracts using 5 μg of Caspase 3 antibody (GTX110543).
Western blot analysis was performed using Caspase 3 antibody (GTX110543).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Caspase 3 antibody detects Caspase 3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Caspase 3 stained by Caspase 3 antibody (GTX110543) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Caspase 3 antibody detects Caspase 3 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Caspase 3 antibody (GTX110543) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Caspase 3 antibody detects Caspase 3 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
12% SDS-PAGE
Caspase 3 antibody (GTX110543) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Caspase 3 antibody detects Caspase 3 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Caspase 3 stained by Caspase 3 antibody (GTX110543) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.
Caspase 3 antibody detects Caspase 3 protein by western blot analysis. Un-treated (-) and treated (+, 30 μM Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验或 C6 cells 作为 cleaved caspase-3 的阳性参照。更多靶点的阳性对照，请登陆CST网站——研究——靶标的阳性对照处理。图二 蛋白质印迹分析（图源自CST Caspase-3 Antibody #9662 抗体说明书）为什么所有的实验都要设置对照？无论是初高中刚接触生物学实验设计还是上研究生后发表 SCI 文章，老师们以及 reviewer 都会敲着「黑板」强调强调再强调，每一个实验都必须有对照！因为设置适当的阴性、阳性甚至空白对照对于实验结果的准确、有效阐释至关重要！阅历丰富
【求助】请问western blot做凋亡检测时，识别caspase-3酶原和激活的caspase-3的抗体是相同的吗？
-caspase-3（#9664）和原形态的caspase-3（#9662），但是需要说明的是，原形态的caspase-3抗体也可以同时检测剪切态的caspase-3，所以建议购买原形态的caspase-3就行了。 （如下图所示） 另外，PARP也是购买全长的就行了，（PARP Antibody #9542）。 lsdcfhyj 谢谢两位的回答 本文由丁香园论坛提供，想了解更多有用的、有意思的前沿资讯以及酷炫的实验
【求助】请教各位师兄，师姐，caspase-3 抗体哪家公司的既便宜又好用啊？
aidiandian 请教各位师兄，师姐，caspase-3 抗体哪家公司的既便宜又好用啊？在下不胜感激··· w_dennis 各位师兄师姐，本人同问该问题，还有caspase-9抗体？ nccunccu cell signalling seagate cst有sample kit，带全了，每样40微升 selenium
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