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- 文献和实验
- 技术资料
- 免疫原:
Full length human Cytochrome C Recombinant protein.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Drosophila, Dog
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX108585
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr
- 浓度:
0.18 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Cytochrome C
- 抗体英文名:
Cytochrome C antibody
- 抗体名:
Cytochrome C 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX108585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX108585) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident pico Western HRP Substrate.
Cytochrome C antibody detects Cytochrome C protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Cytochrome C stained by Cytochrome C antibody (GTX108585) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX108585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX108585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX108585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Sample (30 μg of whole cell lysate)
A: PC-12
15% SDS PAGE
GTX108585 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (50 μg of whole cell lysate)
A: Mouse brain
15% SDS PAGE
GTX108585 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Cytochrome C antibody detects Cytochrome C protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Cytochrome C stained by Cytochrome C antibody (GTX108585) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验广义的细胞色素 c是含有血红素 c的细胞色素之总称。通常是指主要存在于高等动植物、酵母、霉菌等线粒体中的细胞色素 c,起着传递电子的作用。为分子量约 1.3万的碱性蛋白质,还原型的吸收带为 550、 520、 415毫微米,氧化型为 407毫微米。α 因此很早便被研究,并获得结晶。不仅一级结构而且立体结构也已清楚(图 1)。其结构基本上是生物界共有的。由细胞色素 c氧化酶〔细胞色素( a+ a3 )复合体〕氧化。在呼吸链中从细胞色素 c开始接受电子,各种还原酶都已清楚
Cytochrome c Stabilization and Immobilization in Aerogels
structurally intact within the gels during the necessary supercritical fluid processing. However, the heme protein cytochrome c (cyt. c ) forms self-organized superstructures around gold (or silver) nanoparticles in buffer that can be encapsulated within silica
Assessing Cytochrome-c Release from Mitochondria
In recent years substantial evidence has accumulated demonstrating a central regulatory role for mitochondria in cell death (1 ). More recently, mechanisms of cell death, through apoptosis, have been elucidated
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