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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Dengue virus NS5 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Dengue virus
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Dengue virus 2
- 目录编号:
GTX103350
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF
- 浓度:
1.59 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Dengue virus NS5 protein
- 抗体英文名:
Dengue virus NS5 protein antibody
- 抗体名:
Dengue virus NS5 protein 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
NS5 (Dengue virus) antibody detects NS5 (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
7.5% SDS-PAGE
NS5 (Dengue virus) antibody (GTX103350) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with (GTX103350) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX103350 IF Image Immunofluorescence analysis of Denque virus 2-infected BHK-21, using Non-structural protein NS5 (Dengue virus 2 ) antibody (GTX103350) at 1:2000 dilution.
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文献和实验Pan P et al., Signal Transduct Target Ther 2023 (PMID:37160897)
Wu Y et al., Front Microbiol 2022 (PMID:35865923)
Shwetha Shivaprasad et al., PLoS Pathog 2022 (PMID:35482886)
Weng SC et al., Parasit Vectors 2021 (PMID:34311776)
Aviner R et al., Nature 2021 (PMID:34408324)
Marceau CD et al., Nature 2016 (PMID:27383987)
De Jes?s-Gonz?lez LA et al., Viruses 2020 (PMID:32466480)
Roth H et al., MBio 2017 (PMID:28074025)
Zhang J et al., Cell Host Microbe 2018 (PMID:29902443)
Hafirassou ML et al., Cell Rep 2017 (PMID:29281836)
Reyes-Ruiz JM et al., Virology 2017 (PMID:29272748)
Lim SP et al., PLoS Pathog 2016 (PMID:27500641)
Marceau C.D. et al., Nature 2016 (Epub)
Naik NG et al., J Virol 2015 (PMID:25878113)
Fraser JE et al., Methods Mol Biol 2014 (PMID:24696345)
de Wispelaere M et al., J Virol 2013 (PMID:23616652)
The nonstructural protein 5 (NS5) of dengue virus (DENV) plays a central role in the virus replication. It functions as a methyltransferase and an RNA-dependent RNA polymerase. As such, it is a promising target for antiviral drug development
against dengue, timely diagnosis is therefore necessary for patient management. Laboratory diagnosis is carried out by virus isolation, demonstration of viral antigen, presence of viral nucleic acid, and antibodies. Further, recombinant dengue envelope protein
Characterization of the Dengue Virus Envelope Glycoprotein Expressed in Pichia pastoris
The full-length and truncated forms of recombinant envelope (E) glycoprotein from Dengue virus type 1, Singapore strain S275/90 were expressed in the yeast, Pichia pastoris , using a secretory vector. A truncated form of the E protein
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