- ￥5600
- GeneTex
- 美国
- GTX23484
- 2025年07月16日
- WB, ICC/IF, FACS, Gel supershift assays
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic phosphopeptide corresponding to residues I(8) C P L (pS) P L E A D D L(19) of mouse PPAR alpha.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Mouse
- 目录编号：
GTX23484
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, FACS, Gel supershift assays
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
PPAR alpha (phospho Ser12)
- 抗体英文名：
PPAR alpha (phospho Ser12) antibody
- 抗体名：
PPAR alpha (phospho Ser12) 抗体
- 规格：
100 μg
WB analysis of whole cell extracts (20 μg lysate) of U-87 MG (Lane 1), MCF7 (Lane 2), MDA-MB-231 (Lane 3), C2C12 (lane 4), Hep G2 (lane 5) and NIH/3T3 (lane 6) using GTX23484 PPAR alpha (phospho Ser12) antibody.
Dilution : 1:250-1:1000
ICC/IF analysis of C2C12 cells using GTX23484 PPAR alpha (phospho Ser12) antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC
WB analysis of 25 ug of C2C12 (lane 1), NIH-3T3 (lane 2), 3T3-L1 (lane 3) and HepG2 (lane 4) cell lysates using GTX23484 PPAR alpha (phospho Ser12) antibody.
Dilution : 1:200
ICC/IF analysis of U-87 MG cells using GTX23484 PPAR alpha (phospho Ser12) antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC
ICC/IF analysis of 3T3-L1 cells using GTX23484 PPAR alpha (phospho Ser12) antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC
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文献和实验Guo S et al., Brain Res 2019 (PMID:31356784)
PPAR-Alpha Cloning, Expression, and Characterization
Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that also comprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays
In situ hybridisation to alpha satellite sequences (chromosome specific)
anti-mouse HRP antibody diluted 1:80 for 40 minutes at 37C We use DAKO P0260 (12) Wash with blocking solution for 10 minutes (13) Incubate with swine anti-rabbit HRP antibody diluted 1:100 for 40 minutes at 37C We use DAKO P0217 (14
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
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