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Hoechst 33342 是常用的能穿透细胞膜的复染剂,与 DNA 结合是发出蓝色荧光 NucBlue® Live ReadyProbes® Reagen 在室温下稳定,而且是易用的滴管形式,只要挤一下,滴两滴就能给细胞染色。
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文献和实验, so that Gram - cells can be visualized. In practice then, the distinction is made between purple cells (+) and pink cells(-). Determine the basic cell shape of the bacteria. Add the information on Gram stain and cell
Vybrant® DyeCycle Violet Stain
’ instructions, including wash steps. Vybrant® DyeCycle™ Violet stain should be the last stain applied to the sample, and do not wash or fix samples prior to flow cytometric analysis. General Guidelines For optimal DNA content cell cycle
A. Reagents 1. DMEM:10% FBS 2. DMEM:10% FBS:1% HEPES (DFH) 3. 10X Substrate Buffer, pH 6.0 36.6 g Citric Acid, monohydrate 113.5 g Potassium dibasic phosphate Dissolve in 900 ml di-H2O. Check pH and adjust to 6.0 if necessary. Qs
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