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DAPI 是常用的固定细胞核复染剂,与 DNA 结合发出蓝色荧光。通过 NucBlue® Fixed Cell ReadyProbes® Reagent 是高纯度的 DAPI 经典染料,在室温下稳定,而且是易用的滴管形式,只要挤一下,滴两滴就能给细胞染色。
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文献和实验Vybrant® DyeCycle Violet Stain
’ instructions, including wash steps. Vybrant® DyeCycle™ Violet stain should be the last stain applied to the sample, and do not wash or fix samples prior to flow cytometric analysis. General Guidelines For optimal DNA content cell cycle
, so that Gram - cells can be visualized. In practice then, the distinction is made between purple cells (+) and pink cells(-). Determine the basic cell shape of the bacteria. Add the information on Gram stain and cell
Vybrant® DyeCycle Green and Orange Stains
or Vybrant® DyeCycle™ Orange stain from the freezer and allow it to equilibrate to room temperature. Prepare flow cytometry tubes each containing 1 mL of cell suspension in complete media at a concentration of 1 × 106 cells/mL. To each tube
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