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文献和实验reading of 10. For example, this would equal 1 ml if 10 ml of culture at OD600 nm = 1.0 had been centrifuged and resuspended. *** This step is necessary to equalize the amount of cells (and protein) in a given volume of whole cell extract.
Whole Cell Extracts REFERENCE: Hoffman, G., Garrison, T. R., and Dohlman, H. G., Analysis of RGS proteins in Saccharomyces cerevisiae , Methods Enzymol. 344:617-631, 2002. -Use sterile technique and sterile
In Vitro Replication Assay with Mammalian Cell Extracts
Regulatory mechanisms for DNA replication are crucial to the control of the cell cycle in eukaryotic cells. One of the widely used assays to understand the complex mammalian replication system is the cell-free in vitro replication assay (IVRA
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