- 询价
- Cell Signaling Technology
- 2858
- USA
- 2025年11月08日
- W, IHC-P, IF-IC
- Rabbit
- H,M,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
eIF4GI Antibody
- 抗原：
synthetic peptide corresponding to the sequence of human eIF4GI
- 应用范围：
W, IHC-P, IF-IC
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 供应商：
CST
- 库存：
大量
- 适应物种：
H,M,R
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P IF-IC | H M R | Endogenous | 220 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | eIF4GI Antibody detects endogenous levels of total eIF4GI protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human eIF4GI. Antibodies are purified by peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from HepG2, RD and A204 cells using eIF4GI Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using eIF4GI Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using eIF4GI Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lymphoma using eIF4GI Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using eIF4GI Antibody in the presence of control peptide (left) or antigen-specific peptide (right). IF-IC
Confocal immunofluorescent analysis of HeLa cells using eIF4GI Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | The initiation of translation is an important biological event and a variety of factors contribute to this process. Members of the eIF4 translation initiation factor family bind to the 5' m7 GTP mRNA cap and unwind the mRNA secondary structure (1,2). The amino-terminal portion of eIF4G physically associates with eIF4E to stimulate the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A and serves as an adaptor molecule in the eIF4 complex (4). Moreover, eIF4G plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5,6). The eIF4G family includes eIF4G1 (eIF4GI), eIF4G2 (p97, DAP5 or NAT1), and eIF4G3 (eIF4GII) (7). These factors share a homologous sequence that provides for interaction with initiation factors eIF3 and eIF4A. Both eIF4G1 and eIF4G3 are involved in cap-dependent translation, while eIF4G2 plays a role in IRES-mediated translation of some genes during cell stress (7,8).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Following its discovery in rat intestinal epithelial cells (1 ), the peptide YY (PYY) receptor has been characterized in the proximal tubule PKSV-PCT cell line derived from kidneys of SV40 large T antigen transgenic mice
【交流】内质网应激信号通路eIF2a、P-eIF2a Western blot
chengyi1228 各位前辈，我准备做内质网应激通路eIF2a、P-eIF2a两个蛋白的 Western blot。请问谁有这方面经验可以交流一下么？ 对细胞处理后多久提P-eIF2a蛋白比较合适呢？ 谢谢~ batiwangna 内质网应激比较急，具体时间自己摸索，不过建议不要超过24h 辉辉8916 请问你们还做内质网应激的其他通路吗？像ATF6的检测
帮助将抑制因子tRNA交付到核糖体的翻译因子eIF2利用GTP水解的能量来发挥功能。另一个因子eIF5已知在当eIF2与抑制因子tRNA和核糖体结合时会加快eIF2的GTP酶活性。在这项研究中，研究人员确定了eIF5的另外两个作用。 一个涉及在eIF2上稳定GDP（GTP水解的产物）；另一个涉及其通过磷酸化的eIF2来发挥作用、以抑制鸟甙酸交换因子eIF2B。这些结果澄清了我们对翻译抑制怎样被调控的认识。 eIF5 has GDI activity necessary
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