Translation Initiation Complex

Dynabeads® Co-Immunoprecipitation Kit

Co-Immunoprecipitation Optimization below) from which the desired protein complex is isolated. The Extraction Buffer is made with 5 × IP supplied in the kit. The 5 × IP is supplied as 5 × concentrate and in sufficient quantity to allow for 40 coimmunoprecipitation

Ribosomal Initiation Complexes Probed by Toeprinting and Effect of trans-Acting Translational Regulators in Bacteria

and translational factors on the formation of the active ribosomal initiation complex. Moreover, this method offers an easy way to study in vitro how mRNA conformational changes alter ribosome binding at the initiation site. These changes can be induced

Genome‐Wide Annotation and Quantitation of Translation by Ribosome Profiling

reading frames, an adaptation that reveals the sites of translation initiation by pre?treating cells with harringtonine to immobilize initiating ribosomes is described. The protocol described requires 5 to 7 days to generate a completed ribosome profiling