- 询价
- Cell Signaling Technology
- 3680
- USA
- 2025年12月19日
- IHC-P
- H,M,R,Mk,Dm,X,Hr
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Histone H3 (96C10) Mouse mAb (IHC Formulated)
- 抗原：
synthetic peptide corresponding to the carboxy terminus of human histone H3
- 应用范围：
IHC-P
- 库存：
大量
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 适应物种：
H,M,R,Mk,Dm,X,Hr
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (50 sections)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (50 sections) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Dm=D. melanogaster X=Xenopus Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| IHC-P | H (M) (R) (Mk) (Dm) (X) (Hr) | Endogenous | 17 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Histone H3 (96C10) Mouse mAb (IHC Formulated) detects endogenous levels of total histone H3 protein. The antibody does not cross-react with other histone proteins. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human histone H3. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Histone H3 (96C10) Mouse mAb (IHC Formulated). IHC-P (paraffin)
Immunohistochemical analysis of human breast carcinoma using Histone H3 (96C10) Mouse mAb (IHC Formulated)in the presence of control peptide (left) or antigen-specific peptide (right). |
| Background | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验T-Cell Activation Using mAb to CD3
-0031 , or Purified, Cat. No. 14-0031 ) Complete RPMI-1640 Sterile single-cell suspension of mouse spleen or lymph nodes 96-well flat-bottom microtiter plates with lids (Costar Cat. No. 3596) MTT Buffer MTT Lysing Solution
TBS 和 5% NGS 中封闭样品。市售的含有酪蛋白的封闭溶液与磷酸化的一抗结合后易减弱信号；因此，我们建议不使用含有酪蛋白的封闭剂进行磷酸化特异性抗体的检测。以上针对封闭步骤所提建议均是在以 SignalStain. Boost IHC Detection Reagent 作为检测试剂的情况下提出的。如果选择使用其他检测试剂，我们则建议使用与二抗来源相同的血清进行封闭。抗体：Phospho–Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718样本：石蜡
? Boost IHC Detection Reagent (HRP, Rabbit) #8114SignalStain? Boost IHC Detection Reagent (HRP, Mouse) #8125注意: 如果本产品没有使用本试剂优化过，可能需要通过滴定法来确定最佳条件。11. DAB 试剂或合适的底物：按产品说明配制。B. 脱蜡处理/再水化注意：操作中任何时候都不要晾干玻片1. 脱蜡处理/切片的水化：a. 用二甲苯浸洗切片 3 次，每次 5 分钟。b
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