- ￥3870 - 29480
- Sino Biological
- 北京
- 50389-M08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse CD8a / Lyt2 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
50 µg/1 mg
| 规格： | 50 µg | 产品价格： | ￥3870 |
|---|---|---|---|
| 规格： | 1 mg | 产品价格： | ￥29480 |
蛋白名称：CD8 alpha蛋白, CD8 alpha protein
蛋白构建：A DNA sequence encoding the extracellular domain of mouse CD8a (NP_001074579.1) (Met 1-Tyr 196) was expressed, with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1. Measured by its ability to bind biotinylated recombinant human B2M in a functional ELISA. 2. Measured by its ability to bind biotinylated recombinant human FCGRT+B2M in a functional ELISA. 3. Measured by its ability to bind biotinylated recombinant human LCK in a functional ELISA.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Lys 28
蛋白分子量：The secreted recombinant mouse CD8a consists of 180 amino acids and has a calculated molecular mass of 20.3 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 35 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NP_001074579.1
蛋白氨基酸序列：Met1-Tyr196
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验in recombinant Escherichia coli, Biochem. J. 358 (2001) 263-268. 35. G. Lemercier, N. Bakalara, X. Santarelli, On-column refolding of an insoluble histidine tag recombinant exopolyphosphatase from Trypanosoma brucei overexpressed in Escherichia coli, J
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
serumfreeconditioned medium (11) ). 3. Methods When expressing recombinant proteins, the choice of vector hasa major impact on purification strategy, yield, and utility of theproduct. Vectors that link a poly-histidine tag to the recombinant protein typically
) 。 ( 3 ) 384 孔微量滴定板（Gentix，Christchurch，UK ) 。 ( 4 ) 96 孔深孔板：每孔容积 2 ml 的 96 孔微滴定板（Qiagen，Hilden，Germany) 。 ( 5 ) 96 针复制器（Nunc，Wiesbaden，Germany) 。 ( 6 ) 4 X SB 培养基：48 g/L 胰化蛋白胨，96 g/L 酵母提取物，0.8% ( V/V）甘油，120°C 高压灭菌 20 min。 ( 7 ) 20 X PP 缓冲液（磷酸钾盐缓冲
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