- ￥2310 - 25190
- Sino Biological
- 北京
- 11019-H08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human Transferrin / TF Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/50.00 µg/100.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥25190.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥2310.0 |
| 规格： | 100.00 µg | 产品价格： | ￥3870.0 |
蛋白名称：Human Transferrin / TF Protein (His Tag)
蛋白构建：A DNA sequence encoding the human transferrin (NP_001054.1) (Met 1-Pro 698) was fused with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized human CD71 at 10 μg/ml (100 μl/well) can bind human Transferrin. The EC50 of human Transferrin is 5.6 ng/mL. 2. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is typically 0.01-0.04 μg/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Val 20
蛋白分子量：The secreted recombinant human transferrin comprises 690 amino acids with a predicted molecular mass of 76.6 kDa. It migrates as an approximately 74 kDa band in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：NP_001054.1
蛋白氨基酸序列：Met1-Pro698
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Rabbani G, et al. Label-free and ultrasensitive electrochemical transferrin detection biosensor based on a glassy carbon electrode and gold nanoparticles.International journal of biological macromolecules, PubMed ID: 38000589
3, Ahmad A, et al. An affordable label-free ultrasensitive immunosensor based on gold nanoparticles deposited on glassy carbon electrode for the transferrin receptor detection.International journal of biological macromolecules, PubMed ID: 38866289
Chromatin Interaction Analysis Using Paired‐End Tag Sequencing
Analysis using Paired?End Tag sequencing (ChIA?PET) is a technique developed for large?scale, de novo analysis of higher?order chromatin structures. Cells are treated with formaldehyde to cross?link chromatin interactions, DNA segments bound by protein
beads）结合细胞（刀豆蛋白 A 能与细胞膜上的糖蛋白结合）。使用非离子去污剂洋地黄皂苷进行细胞膜通透。然后分步孵育靶蛋白（如转录因子, TF）的抗体（一抗）、二抗、和 Protein A-Tn5 ，抗体和 Protein A-Tn5 能够通过细胞膜、核孔进入细胞核，Tn5 通过 Protein A 和抗体的介导切割靶蛋白附近的 DNA 序列。使用 Tn5 在进行切割的时候，会在被切割的片段两端加上接头序列，通过 PCR 扩增直接可以得到二代测序文库。 图 9: CUT&Tag、CUT
.3 ). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA?binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA?binding
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