Recombinant Human EPOR / Erythropoietin Receptor Protein (Fc Tag) | 重组人 EPOR / Erythropoietin Receptor 蛋白 (Fc标签)

重组人 EPOR / Erythropoietin Receptor 蛋白 (Fc标签)（产品说明）
蛋白名称：Human EPOR / Erythropoietin Receptor Protein (Fc Tag)
蛋白构建：A DNA sequence encoding the extracellular domain (Met-Pro 250) of human erythropoietin receptor (NP_000112.1) precursor was expressed with the C-terminal fused Fc region of human IgG1.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized EPOR (hFc Tag) (Cat:10707-H02H) at 2μg/mL (100μL/well) can bind Cynomolgus Erythropoietin-His (Cat:90133-C08H), the EC50 of Cynomolgus Erythropoietin-His (Cat: 90133-C08H) is 4-20 ng/mL. 2. Measured by its ability to inhibit Epo­dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 1-4 ng/ml in the presence of 0.1 U/mL Recombinant Human EPO.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ala 25
蛋白分子量：The recombinant human EPOR/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 460 amino acids and has a predicted molecular mass of 51.0 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEPOR/Fc monomer is approximately 55-60 kDa due to glycosylation.
蛋白NP号：NP_000112.1
蛋白氨基酸序列：Met1-Pro250
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.