- ￥580 - 22680
- Sino Biological
- 北京
- 50349-MNAE
- 2025年08月12日
企业认证
相关产品推荐更多 >
LYVE1重组蛋白|Recombinant Mouse LYVE1 / LYVE-1 Protein (Fc Tag)
￥1790P-Selectin/CD62P重组蛋白|Recombinant Mouse SELP / selectin P / P-selectin Protein (His Tag)
￥1400
HER3/ERBB3重组蛋白|Recombinant Mouse HER3/ERBB3 Protein (ECD,His & AVI Tag), Biotinylated
￥3870Mesothelin重组蛋白|Recombinant Mouse Mesothelin Protein (His Tag)
￥3870
CAMKI重组蛋白|CAMK1 Protein, Active
￥3480
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse TNF-alpha / TNFA Protein (ECD)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/5.00 µg/20.00 µg/100.00 µg/500.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥22680.0 |
|---|---|---|---|
| 规格： | 5.00 µg | 产品价格： | ￥580.0 |
| 规格： | 20.00 µg | 产品价格： | ￥1380.0 |
| 规格： | 100.00 µg | 产品价格： | ￥3480.0 |
| 规格： | 500.00 µg | 产品价格： | ￥14480.0 |
蛋白名称：Mouse TNF-alpha / TNFA Protein (ECD)
蛋白构建：A DNA sequence encoding the soluble form of mouse TNF-α (NP_038721.1) (Leu 80-Leu 235) was expressed, with an initial Met at the N-terminus.
表达宿主：E. coli
蛋白纯度：> 98 % as determined by SDS-PAGE
蛋白活性：Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.
蛋白内毒素：Please contact us for more information.
预测N端：Met
蛋白分子量：The recombinant mouse TNF-α consists of 157 amino acids and migrates with an apparent molecular mass of 17.39 kDa as estimated in SDS-PAGE under reducing conditions.
蛋白NP号：NP_038721.1
蛋白氨基酸序列：Leu80-Leu235
蛋白标签：Native
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验2, Ji KL, et al. Alpinia hainanensis Rhizome Extract Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis: Active Ingredient Investigation and Evaluation.Journal of agricultural and food chemistry, PubMed ID: 35321548
3, Yu Y, et al. Repurposing crizotinib to target RIPK1-dependent cell death.International immunology, PubMed ID: 36534968
4, Yu L, et al. Repositioning linifanib as a potent anti-necroptosis agent for sepsis.Cell death discovery, PubMed ID: 36765040
5, He X, et al. Identification of Piperlongumine as Potent Inhibitor of Necroptosis.Drug design, development and therapy, PubMed ID: 37188283
6, Li M, et al. Quizartinib inhibits necroptosis by targeting receptor-interacting serine/threonine protein kinase 1.FASEB journal : official publication of the Federation of American Societies for Experimental Biology, PubMed ID: 37698367
7, Lin J, et al. IL-33 promotes pancreatic β-cell survival and insulin secretion under diabetogenic conditions through PPARγ.European journal of pharmacology, PubMed ID: 37758011
8, Yan T, et al. Single-cell transcriptomic analysis reveals rich pituitary-Immune interactions under systemic inflammation.PLoS biology, PubMed ID: 38109308
9, Hou S, et al. PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis.Molecular cell, PubMed ID: 38272024
10, Qiu J, et al. PI3Kδ inhibition alleviates the brain injury during cerebral ischemia reperfusion via suppressing pericyte contraction in a TNF-α dependent manner.Experimental neurology, PubMed ID: 38365134
11, Wang D, et al. Targeting TNF-α: The therapeutic potential of certolizumab pegol in the early period of cerebral ischemia reperfusion injury in mice.International immunopharmacology, PubMed ID: 38908079
Intracellular Cytokine Staining Protocol
-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used.Note: Optimal
Intracellular Cytokine Staining Protocol
to produce IFN-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used
overnight. Read plate at 570-650 nm. Graph standard curve and analyze data. Protocol B: TNF-alpha-Induced Killing of L929 Cell Line What You Need Materials L929 mouse fibroblast line (ATCC Cat. No. CCL-1)
技术资料暂无技术资料 索取技术资料
