- ￥580 - 22680
- Sino Biological
- 北京
- 50349-MNAE
- 2026年01月26日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse TNF-alpha / TNFA Protein (ECD)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/5.00 µg/20.00 µg/100.00 µg/500.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥22680.0 |
|---|---|---|---|
| 规格： | 5.00 µg | 产品价格： | ￥580.0 |
| 规格： | 20.00 µg | 产品价格： | ￥1380.0 |
| 规格： | 100.00 µg | 产品价格： | ￥3480.0 |
| 规格： | 500.00 µg | 产品价格： | ￥14480.0 |
蛋白名称：Mouse TNF-alpha / TNFA Protein (ECD)
蛋白构建：A DNA sequence encoding the soluble form of mouse TNF-α (NP_038721.1) (Leu 80-Leu 235) was expressed, with an initial Met at the N-terminus.
表达宿主：E. coli
蛋白纯度：> 98 % as determined by SDS-PAGE
蛋白活性：Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.
蛋白内毒素：Please contact us for more information.
预测N端：Met
蛋白分子量：The recombinant mouse TNF-α consists of 157 amino acids and migrates with an apparent molecular mass of 17.39 kDa as estimated in SDS-PAGE under reducing conditions.
蛋白NP号：NP_038721.1
蛋白氨基酸序列：Leu80-Leu235
蛋白标签：Native
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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- 作者
- 内容
- 询问日期
文献和实验2, Ji, KL; et al. Alpinia hainanensis Rhizome Extract Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis: Active Ingredient Investigation and Evaluation. Journal of agricultural and food chemistry, PMID: 35321548
3, Yu, Y; et al. Repurposing crizotinib to target RIPK1-dependent cell death. International immunology, PMID: 36534968
4, Yu, L; et al. Repositioning linifanib as a potent anti-necroptosis agent for sepsis. Cell death discovery, PMID: 36765040
5, He, X; et al. Identification of Piperlongumine as Potent Inhibitor of Necroptosis. Drug design, development and therapy, PMID: 37188283
6, Lin, J; et al. IL-33 promotes pancreatic β-cell survival and insulin secretion under diabetogenic conditions through PPARγ. European journal of pharmacology, PMID: 37758011
7, Li, M; et al. Quizartinib inhibits necroptosis by targeting receptor-interacting serine/threonine protein kinase 1. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, PMID: 37698367
8, Yan, T; et al. Single-cell transcriptomic analysis reveals rich pituitary-Immune interactions under systemic inflammation. PLoS biology, PMID: 38109308
9, Hou, S; et al. PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis. Molecular cell, PMID: 38272024
10, Qiu, J; et al. PI3Kδ inhibition alleviates the brain injury during cerebral ischemia reperfusion via suppressing pericyte contraction in a TNF-α dependent manner. Experimental neurology, PMID: 38365134
11, Wang, D; et al. Targeting TNF-α: The therapeutic potential of certolizumab pegol in the early period of cerebral ischemia reperfusion injury in mice. International immunopharmacology, PMID: 38908079 1
2, Tao, X; et al. Astrocyte-conditional knockout of MOB2 inhibits the phenotypic conversion of reactive astrocytes from A1 to A2 following spinal cord injury in mice. International journal of biological macromolecules, PMID: 39863205 1
3, Zhang, N; et al. Palmitoylation licenses RIPK1 kinase activity and cytotoxicity in the TNF pathway. Molecular cell, PMID: 39471814 1
4, Chen, Z; et al. RIPK1 senses S-adenosylmethionine scarcity to drive cell death and inflammation. Cell metabolism, PMID: 40570842
近年来，重组蛋白的应用有了显著增长，与此同时，用于重组蛋白表达和纯化的技术和产品也得以增长。自从亲和标签融合技术出现以来，多种多样的短肽或蛋白亲和标签极大地方便了外源表达重组蛋白的分离与蛋白质复合体的纯化，已成为蛋白质研究领域不可或缺的工具。多聚组氨酸标签（His-tag）是目前高通量蛋白纯化最普遍使用的亲和标签，His 标签融合蛋白的固定化金属离子亲和层析（immobilized metal-ion affinity chromatography, IMAC）被作为主要的蛋白纯化方法。从重组蛋白
re-loxP 是一种位点特异的基因重组技术，被广泛应用于特异位点的基因敲除、基因插入、基因翻转和基因易位，在真核生物和原核生物中均有广泛应用。Cre-loxP 的基本原理Cre 蛋白是一种重组酶，是causes recombination的缩写，于1981 年从 P1 噬菌体中被发现，属于 λInt 酶超基因家族。Cre 重组酶基因编码区序列全长1029bp（EMBL 数据库登录号为 X03453），编码 38kDa 蛋白质，Cre 重组酶是由 343 个氨基酸组成的单体蛋白。LoxP 位点
肝炎病毒包膜E2蛋白相同的亲和结合力3。另外，有阴离子转运载体AE1结构域的重组蛋白被标记且用Rho1D4系统纯化后表现出与红细胞来源的AE1蛋白有相同的硫酸外排速率。4） 利用Rho1D4纯化膜蛋白图解简述步骤1：结合：将Rho1D4标记的蛋白与载有Rho1D4抗体的琼脂糖一同孵育使得目标蛋白被固定在吸附材料上。 步骤2：清洗：将杂蛋白和其他裂解液成分洗去，留下与亲和基质结合的目标蛋白。 步骤3：洗脱：过量Rho1D4肽与基质竞争性结合，释放出目标蛋白在洗脱液中收集。 表
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