Carboxypeptidase E/CPE重组蛋白|Recombinant Human Carboxypeptidase E / CPE Protein (His Tag)
- ￥1790 - 3870
- Sino Biological
- 北京
- 10069-H08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human Carboxypeptidase E / CPE Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
10.00 µg/20.00 µg/50.00 µg
| 规格： | 10.00 µg | 产品价格： | ￥1790.0 |
|---|---|---|---|
| 规格： | 20.00 µg | 产品价格： | ￥3220.0 |
| 规格： | 50.00 µg | 产品价格： | ￥3870.0 |
蛋白名称：Human Carboxypeptidase E / CPE Protein (His Tag)
蛋白构建：A DNA sequence encoding the human carboxypeptidase E (CPE) precursor (NP_001864.1) (Met 1-Ser 453) was fused with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to cleave a peptide substrate, benzoyl-AR-OH. The product, Arg, reacted with orthophthaldialdehyde (OPA) to form a fluorescent molecule . The specific activity is >12,000 pmoles/min/μg.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Glu 26
蛋白分子量：The recombinant human CPE comprises 439 amino acids and predicts a molecular mass of 49.4 kDa. As a result of glycosylation, the apparent molecular mass of rhCPE is approximately 53 kDa in SDS-PAGE under reducing conditions.
蛋白NP号：NP_001864.1
蛋白氨基酸序列：Met1-Ser453
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Construction and Characterization of Adenovirus Vectors
for cytopathic effect (CPE). The cells should have a rounded morphology or be detached from the plate. 32. Use one plate for analysis of the recombinant Ad structure. Reserve the contents of the other plate for vector expansion
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
On the Discovery of Precursor Processing
. Most neuroendocrine peptides are processed by two specialized members of this family – PC2 and/or PC1/3 – followed by carboxypeptidase E (CPE). This brief report concentrates mainly on the role of insulin biosynthesis in providing a useful early paradigm of precursor
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