- ￥3870
- Sino Biological
- 北京
- 10545-H08H
- 2025年08月12日
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- 详细信息
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Human TFPI2 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
50 µg
重组人 TFPI2 蛋白 (His标签)（产品说明）
蛋白名称：Human TFPI2 Protein (His Tag)
蛋白构建：A DNA sequence encoding the human TFPI2 (NP_006519.1) precursor (Met 1-Lys 213) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114) . The IC50 value is < 2 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15M NaCl , 0.05% Brij-35, pH 7.5.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Asp 23
蛋白分子量：The secreted recombinant humanTFPI2 comprises 202 amino acids and predicts a molecular mass of 23.2 kDa. It migrates as an approximately 32-34 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_006519.1
蛋白氨基酸序列：Met1-Lys213
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白名称：Human TFPI2 Protein (His Tag)
蛋白构建：A DNA sequence encoding the human TFPI2 (NP_006519.1) precursor (Met 1-Lys 213) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114) . The IC50 value is < 2 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15M NaCl , 0.05% Brij-35, pH 7.5.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Asp 23
蛋白分子量：The secreted recombinant humanTFPI2 comprises 202 amino acids and predicts a molecular mass of 23.2 kDa. It migrates as an approximately 32-34 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_006519.1
蛋白氨基酸序列：Met1-Lys213
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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重组人 TFPI2 蛋白 (His标签)
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