- ￥2310 - 3870
- Sino Biological
- 北京
- 10762-H03H
- 2025年08月12日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human EphB2 Protein (His & Fc Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
50.00 µg/100.00 µg
| 规格： | 50.00 µg | 产品价格： | ￥2310.0 |
|---|---|---|---|
| 规格： | 100.00 µg | 产品价格： | ￥3870.0 |
蛋白名称：EphB2蛋白, EphB2 protein
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-Leu 543) of human EphB2 (NP_059145.2) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to bind recombinant human EphrinB2 in a functional ELISA.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Val 19
蛋白分子量：The recombinant human EphB2/Fc is a disulfide-linked homodimer. The reduced monomer consists of 773 amino acids and has a predicted molecular mass of 86 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EphB2/Fc monomer is approximately 90-100 kDa due to glycosylation.
蛋白NP号：NP_059145.2
蛋白氨基酸序列：Met1-Leu543
蛋白标签：C-human IgG1-Fc & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验【原创】Protein A Sepharose 之进化篇。。。
洗脱条件比较剧烈，最后收集的蛋白质很有可能变性，或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域：E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域，仅由5个结构域组成，每个域均可以和IgG的Fc段结合，但不同的域结合强度略有差异。因此洗脱条件不均一，而且经常需要较低的pH值。如果减少串联结构域的个数，并且采取同型结构域串联，就可以避免不同结构域与抗体Fc 段亲和性的差异，洗脱条件会温和而均一。为此，我们可以做一个试验，比较一下
Using the scan‐x Web Site to Predict Protein Post‐Translational Modifications
spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post?translational modification. We describe a protocol to use the scan?x Web site to view predicted acetylation sites in the human proteome and predicted
Protein Components of the microRNA Pathway and Human Diseases
, Exportin-5 (Exp-5), TAR RNA binding protein (TRBP) and fragile X mental retardation protein (FMRP), are each related, in various ways, to specific genetic diseases.
技术资料暂无技术资料 索取技术资料
