重组人 VEGFR1 / FLT-1 蛋白 (Fc标签)(产品说明)
蛋白名称:Human VEGFR1 / FLT-1 Protein (Fc Tag)
蛋白构建:A DNA sequence encoding the human FLT1 (NP_001153503.1) (Met1-Ile328) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:1. Measured by its binding ability in a functional ELISA. Immobilized human VEGFR1-Fc(Cat:10136-H02H) at 10μg/mL (100μL/well) can bind biotinylated human VEGF165 (Cat:11066-HNAH), the EC50 of biotinylated human VEGF165 is 10-40 ng/mL.2. Measured by its ability to inhibit VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 20-100 ng/mL in the presence of 10 ng/mL rhVEGF165.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Ser 27
蛋白分子量:The recombinant human FLT1 consists 543 amino acids and predicts a molecular mass of 61.1 kDa.
蛋白NP号:NP_001153503.1
蛋白氨基酸序列:Met1-Ile328
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.