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100 µg
蛋白名称:CAR/CXADR蛋白, CAR/CXADR protein
蛋白构建:A DNA sequence encoding the mouse CXADR (NP_001020363.1) extracellular domain (Met 1-Gly 237) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 92 % as determined by SDS-PAGE
蛋白活性:Measured by the ability of the immobilized protein to support the adhesion of mouse neutrophils. When 5 x 10E4 cells/well are added to CXADR-coated plates (4 μg/ml and 100 μl/well), approximately 20%-40% will adhere specifically after 60 minutes at 37℃.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Leu 20
蛋白分子量:The recombinant mouse CXADR/Fc is a disulfide-linked homodimer. The reduced monomer consists of 466 amino acids and has a predicted molecular mass of 52 kDa. As a result of glycosylation, the apparent molecular mass of rm CXADR/Fc monomer migrates with an apparent molecular mass of 60-65 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:NP_001020363.1
蛋白氨基酸序列:Met1-Gly237
蛋白标签:C-human IgG1-Fc & His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Using the scan‐x Web Site to Predict Protein Post‐Translational Modifications
spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post?translational modification. We describe a protocol to use the scan?x Web site to view predicted acetylation sites in the human proteome and predicted
-T 靠谱么?瘤内注射还是局部注射?什么时间检测 CAR-T 杀伤效果?怎么检测 CAR-T的体内增殖?长效检测需要做多久?【第十坑】你的时间耗得起吗?吃苦耐劳、动手能力逆天的熊主任克服了一个又一个技术坑,终于排除万难完成了肿瘤靶点 X 的CAR-T 临床前实验,拿到阳性结果的老熊却一点儿也开心不起来,三年!这个项目足足耗了三年时间!这三年,熊主任的头顶日渐稀疏,憔悴的汪博士一再延期……最要命的是,相同靶标的CAR-T 文章已经被抢发了,隔壁科室的老王只花了一年时间抢先完成了另一种靶标的临床前实验
Nature:超级 CAR-T 细胞可完全消灭肿瘤,小鼠存活率达 100%
当前,上市的细胞免疫疗法都是针对血液肿瘤的,对于实体肿瘤而言, CAR-T仍然还面临许多困境:CAR-T细胞无法有效地进入实体瘤组织内部,导致无法与肿瘤细胞直接接触进而消灭肿瘤。2018年3月5日,国际著名学术期刊《nature biotechnology》在线发表了一篇关于实体肿瘤研究的最新突破:在传统CAR-T基础之上添加IL-17以及CCL19这两个基因进行改造,变成超级CAR-T细胞(科学家将其命名为7x19CAR-T),能够有效杀伤肿瘤,这种细胞在体内存活时间更长并且能够有效地帮助
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