Influenza A H1N1 Neuraminidase / NA (His Tag)

Influenza A H1N1 (A/California/04/2009) Neuraminidase / NA (His Tag): 产品信息
纯度> 88 % as determined by SDS-PAGE
内毒素< 1.0 EU per μg of the protein as determined by the LAL method
生物活性Testing in progress
蛋白构建A DNA sequence encoding the mature form of influenza A virus (A/California/04/2009 (H1N1)) neuraminidase (ACP41107.1) (His 36-Lys 469) was fused with a polyhistidine tag at the N-terminus.
Accession#ACP41107.1
表达宿主Baculovirus-Insect Cells
种属H1N1
预测 N 端His
分子量The recombinant influenza H1N1 virus neuraminidase (A/California/04/2009 (H1N1)) comprises 450 amino acids with the predicted molecular mass of 50 kDa. The apparent molecular mass of the recombinant protein is approximately 55 kDa in SDS-PAGE under reducing conditions.
缓冲液Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hardcopy of datasheet.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Influenza A H1N1 (A/California/04/2009) Neuraminidase / NA (His Tag): 图片


Influenza A H1N1 (A/California/04/2009) Neuraminidase / NA (His Tag): 别称
NA Protein, H1N1

Neuraminidase/NA 背景信息
Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.