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- 细胞类型：
B cell precursor leukemia
- 物种来源：
human
DSMZ no.:ACC 546
Species:human (Homo sapiens)
Cell type:B cell precursor leukemia
Origin:
established from the peripheral blood of a 5-year-old girl at relapse of acute lymphoblastic leukemia (ALL) in 1990; reported to carry the t(4;11) MLL-AFF1 (MLL-AF4)
Biosafety level:1
Permissions and restrictions:A
DSMZ Cell Culture Data:
Morphology: round to polygonal single rather small cells in suspension; image;image
Medium: 90% Iscove's MDM + 10% h.i. FBS
Subculture: split saturated culture 1:2 to 1:6 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-2.0 x 106 cells/ml; maximum density at ca. 3-4 x 106 cells/ml
Incubation: at 37 °C with 5% CO2
Doubling time: ca. 30 hours
Harvest: cell harvest of ca. 3-4 x 106 cells/ml
Storage: frozen with 70% medium, 20% FBS, 10% DMSO
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