- ¥658
- 优碧生物
- 中国
- UBM-K48C
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 英文名：
Ubiquitin Mutant K48C
- 供应商：
优碧生物
- 规格：
1mg
Mutation of lysine 48 to cystine renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 48 linkages with other Ub molecules. Ub K48C can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins (monoubiquitination). Ideal for the reduction in poly-Ub chain length/conjugation rates and determining if poly-Ub chains are K48 linked.
- MW: 8.5 kDa
- Concentration: 2mg/mL
- Buffer: 50mM PBS, pH7.0, 150mM NaCl
- Solubility: Aqueous solutions up to 50 mg/ml
- Purity: > 95% by SDS‐PAGE
- Storage: Store at ‐20℃. Avoid multiple FREEZE/THAW cycles.
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| 说明书（datasheet） |
优碧生物产品可以通过本公司、电话、传真、E-mail或授权（区域）经销商等方式订购。
电话: 18943659183 （订购电话）
传真: （+86）0431-82632000
Email:ubbiotech (技术支持）
QQ：1743024340（售前，售后，技术支持）
网站:
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文献和实验C. elegans Deletion Mutant Screening
The methods used by the Caenorhabditis elegans Gene Knockout Consortium are conceptually simple. One does a chemical mutagenesis of wild-type C. elegans , and then screens the progeny of the mutagenized animals, in small mixed groups, using
Several important signaling processes depend on the tagging of cellular proteins with “polyubiquitin chains”—ubiquitin polymers whose building blocks are connected by isopeptide bonds between G76 of one ubiquitin and a specific lysine residue
Studies of the Ubiquitin Proteasome System
6.3 ) Alternate Protocol 2: Determination of Ubiquitin Chain Variant Phenotype 20× ubiquitylation buffer 2 for E3 assays (see recipe ) Mutant ubiquitins: lysine‐less (K0), K48R, K63R, K48 only, K63 only (BioMol
