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- Megazyme
- 爱尔兰
- K-CELLG5
- 2025年12月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4℃
- 保质期：
>1年
- 英文名：
Cellulase Assay Kit
- 库存：
请咨询
- 供应商：
上海经科化学科技有限公司
- 规格：
120/ 240 assays
纤维素酶检测试剂盒介绍：
中文名称：纤维素酶检测试剂盒[CELLG5法]
英文名称：Cellulase Assay Kit (CELLG5 Method)
货号：K-CELLG5
规格：120 / 240 assays (manual) / 480 (auto-analyser)
价格：7237元
类别：检测分析试剂盒
简介：Megazyme检测试剂盒，适用范围广的行业，包括食品，饲料，发酵，酿造，葡萄酒和乳制品，果汁饮料。
| Content: | (K-CellG5-4V) 120 / 240 assays (manual) / 480 assays (auto-analyser) or (K-CellG5-2V) 60 / 120 assays (manual) / 240 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | endo-Cellulase |
| Assay Format: | Spectrophotometer, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Signal Response: | Increase |
| Limit of Detection: | 1.2 x 10-3 U/mL |
| Reproducibility (%): | ~ 3% |
| Total Assay Time: | 10 min |
| Application examples: | Fermentation broths, industrial enzyme preparations and biofuels research. |
| Method recognition: | Novel method |
- Very cost effective
- All reagents stable for > 4 years
- Completely specific for cellulase (endo-1,4-glucanase)
- Generally applicable and highly sensitive
- Simple format. Well suited to automation
- Standard included
Cellulase Activity Assay Kit.
The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
The CellG5 assay represents a huge step forward in the methodology for the measurement of cellulase that traditionally relied on substrates such as CM-cellulose, Avicel, cellooligosaccharides, filter paper or dyed polysaccharides including CMC Congo red or cellulose azure.
View Cellulase Activity Assay Protocol.
View our complete list of assay kits for enzyme activities.
纤维素酶检测试剂盒说明书(PDF)请向客服索取
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