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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
> 4 years at 4oC.
- 英文名:
α-L-Arabinofuranosidase (A. niger)
- 库存:
请咨询
- 供应商:
上海经科化学科技有限公司
- 保存条件:
2-8℃
- 规格:
480U
中文名称:α-L-阿拉伯呋喃糖酶[黑曲菌]
英文名称:α-L-Arabinofuranosidase (Asper-gillus niger)
货号:E-AFASE
规格:480 Units
价格:3570元
类别:碳水化合物活性酶
High purity α-L-Arabinofuranosidase (Asper-gillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Content: | 480 Units on pNP-α-Arabf at 40oC |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | α-Arabinofuranosidase |
| EC Number: | 3.2.1.55 |
| CAZy Family: | GH51 |
| CAS Number: | 9067-74-7 |
| Synonyms: | non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase |
| Source: | Asper-gillus niger |
| Molecular Weight: | 62,000 |
| Concentration: | Supplied at ~ 300 U/mL |
| Expression: | From Asper-gillus niger |
| Specificity: | Hydrolysis of α-1,2- and α-1,3-linked L-arabinofuranose residues from arabinoxylans and branched arabinans. Hydrolyses α-1,5-linked arabino-oligosaccharides at a much lower rate. |
| Specific Activity: | ~ 32 U/mg (40oC, pH 5.5 on p-nitrophenyl-α-L-arabinofuranoside); ~ 12.6 U/mg (on sugar-beet arabinan); ~ 1.2 U/mg (on wheat arabinoxylan) |
| Unit Definition: | One Unit of α-L-arabinofuranosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-L-arabinofuranoside (5 mM) in sodium acetate buffer (100 mM), pH 4.0 at 40oC. |
| Temperature Optima: | 40oC |
| pH Optima: | 4 |
| Application examples: | Applications in carbohydrate and biofuels research. |
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文献和实验1.Cloning of an α-L-Arabinofuranosidase and Characterization of Its Action on Mono-and Di-Substituted Xylopyranosyl Units.
Wong, D. W. & Batt, S. (2022). Advances in Enzyme Research, 10(4), 75-82.
2.A conserved enzyme of smut fungi facilitates cell-to-cell extension in the plant bundle sheath.
Ökmen, B., Jaeger, E., Schilling, L., Finke, N., Klemd, A., Lee, Y. J., Wemhoner, R., Pauly, M., Neumann, U. & Doehlemann, G. (2022). Nature Communications, 13(1), 1-13.
ara(Arabinose) 功能:ara基因表达阿拉伯糖代谢所需的各种酶,包括: araA表达阿拉伯糖异构酶、 araB表达核酮糖激酶、araC表达阻遏蛋白(起调节作用), araD表达 L-核酮糖-4-磷酸差向异构酶、araE表达低亲和型 L-阿拉伯糖转运蛋白、araF表达 L-阿拉伯糖结合蛋白、araG表达高亲和性的 L-阿拉伯糖转运蛋白。ara基因的变异,使细胞不能利用阿拉伯糖进行能量代谢,可以利用此特性进行菌株筛选。 mtl(Mannitol) 功能
完全在一个平面上,而是以较稳定的椅型构象存在。因此,为了更合理地反映其结构,现在常用构象式来表示。α-D-和β-D-葡萄糖的构象式如下: α-D- 吡喃葡萄糖除C-1上的-OH连在α键上,其它三个碳上的-OH和-CH2OH都连在e键上,而β-D吡喃葡萄糖C-1上的-OH及所有比较大的原子团(-OH,-CH2OH)都连在e键上,因而β型的构象更为稳定。故在溶液中达到平衡时,β型占64%,而α型仅占36%。 二、单糖的性质 (一)物理
形式的 N-聚糖。植物复杂形式 N-糖苷有一个挂在中心的近端氨基葡糖上的 α-1-3 岩藻糖;一个与中心少甘露糖连接的 β-1-2 木糖残基,和/或与它们的末端天线(称为 Lewisa ) 连接的 Gal/β-3 (Fucα-1-4 ) GlcNAc 寡聚糖末端序列,称为 Lewisa ( 图 25-1B ) [1] 。 1. 2 O-糖基化 在植物细胞中 O-糖基化可分为两种类型。即分泌蛋白糖基化或细胞质/核蛋白糖基化。蛋白质上不同位点的 O-连聚糖的性质不同










